Promiscuous interaction of titanium dioxide nanoparticles leads to alterations in structural stability and interferes with luciferase folding

The immense applications of titanium dioxide nanoparticles (TiO 2 NPs) in consumer products have raised concerns about their health effects especially because TiO 2 NPs can interact with various biomolecules. Out of these, proteins are more prone to alterations owing to their dynamic nature. Differe...

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Bibliographic Details
Published in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2023-08, Vol.25 (8), p.163, Article 163
Main Authors: Jaiswal, Surabhi, Verma, Rahul, Mehrotra, Srishti, Patnaik, Satyakam, Pandey, Alok K., Priya, Smriti, Sharma, Sandeep K.
Format: Article
Language:English
Subjects:
Biomolecules
Characterization and Evaluation of Materials
Chemistry and Materials Science
Consumer products
Inorganic Chemistry
Lasers
Materials Science
Nanoparticles
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Proteins
Research Paper
Structural stability
Therapeutic applications
Titanium
Titanium dioxide
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Summary:The immense applications of titanium dioxide nanoparticles (TiO 2 NPs) in consumer products have raised concerns about their health effects especially because TiO 2 NPs can interact with various biomolecules. Out of these, proteins are more prone to alterations owing to their dynamic nature. Different protein conformers co-exist in cellular systems, and studying the interaction of protein conformers with nanoparticles becomes crucial for mechanistic understanding and therapeutic applications of TiO 2 NPs. In the present study, the effect of TiO 2 NPs on native, unfolded, and misfolded luciferase has been investigated. TiO 2 NPs were found to destabilize native luciferase, interfere with the spontaneous refolding of unfolded luciferase, and promote luciferase misfolding; however, minor structural alterations were observed indicating weak interactions between the TiO 2 NPs and luciferase. Morphological analysis of TiO 2 NPs showed an average size of 24.57 ± 4.94 nm that increased to 50.52 ± 16.71 nm upon adsorption of luciferase on to TiO 2 NPs. This study provides an understanding on the implication of nanoparticle-protein interaction on structural and conformational changes in various protein conformers.
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-023-05765-8