Effects of Saccharina japonica Holdfast Powder on Microbiota in the Caecum of Mice Fed a High-Sucrose and Low-Fibre Diet and in Human Faecal Cultures

The holdfast of Saccharina japonica (SjH) is a by-product of kombu cultivation and is rich in dietary fibres and phenolic compounds. SjH is regarded as a functional food material with anti-infection, immunomodulatory, and anti-viral effects. In this study, we aimed to examine SjH-responsive indigeno...

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Published in:Waste and biomass valorization 2023-11, Vol.14 (11), p.3539-3552
Main Authors: Midorikawa, Yuko, Kuda, Takashi, Xia, Yumeng, Nishizawa, Makoto, Yamagishi, Takashi, Takahashi, Hajime, Lee, Gayang
Format: Article
Language:English
Subjects:
Acetic acid
Antiviral agents
Bacteria
Culture
Diet
Dietary fiber
Engineering
Environment
Environmental Engineering/Biotechnology
Feces
Fibers
Functional foods & nutraceuticals
Immunomodulation
Industrial Pollution Prevention
Intestinal microflora
Microbiota
Microorganisms
Nutrient deficiency
Organic acids
Original Paper
Phenolic compounds
Phenols
Renewable and Green Energy
rRNA 16S
Saccharina japonica
Sucrose
Waste Management/Waste Technology
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Summary:The holdfast of Saccharina japonica (SjH) is a by-product of kombu cultivation and is rich in dietary fibres and phenolic compounds. SjH is regarded as a functional food material with anti-infection, immunomodulatory, and anti-viral effects. In this study, we aimed to examine SjH-responsive indigenous gut bacteria (SjH-RIB) in Institute of Cancer Research (ICR) mice fed a diet containing either no fibre or 5% (w w −1 ) SjH for 14 days. Additionally, we sought to determine the effects of SjH on organic acids, putrefactive compounds, and microbiota in human faecal cultures. Human stool samples collected from five healthy volunteers were incubated without (control) or with 2% (w w −1 ) SjH at 37 °C for 24 h. The microbiota in murine caecal samples and human faecal cultures were analysed via 16S rRNA (V4) amplicon sequencing. Compared to the mice fed no-fibre diets, mice fed SjH showed high caecal levels of Clostridium sensu stricto 1 -, Oscillospiraceae spp.-, Parabacteroides spp.-, and Alistipes spp.-like bacteria. In contrast, Allobaculum , a dominant genus in the no-fibre group, was not detected in the SjH group. In human faecal cultures, the levels of acetate and propionate were higher in SjH-cultures than in the control cultures ( p  
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-023-02064-0