Optimization of biomass saccharification processes with experimental design tools for 2G ethanol production: a review

The current energy scenario has encouraged the replacement of carbon sources with renewable sources. Second‐generation (2G) ethanol production from lignocellulosic material or agroindustrial biomass involves a three‐step process consisting of pretreatment, saccharification and fermentation. Enzymati...

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Bibliographic Details
Published in:Biofuels, bioproducts and biorefining bioproducts and biorefining, 2023-11, Vol.17 (6), p.1789-1806
Main Authors: Bernardi, Aline V., Gerolamo, Luis E., Dinamarco, Taisa Magnani, Tapia‐Blácido, Delia Rita
Format: Article
Language:English
Subjects:
Biomass
Carbon sources
Design
Design of experiments
Ethanol
Experimental design
Fermentation
Lignocellulose
Optimization
Pretreatment
Response surface methodology
Saccharification
Substrates
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Summary:The current energy scenario has encouraged the replacement of carbon sources with renewable sources. Second‐generation (2G) ethanol production from lignocellulosic material or agroindustrial biomass involves a three‐step process consisting of pretreatment, saccharification and fermentation. Enzymatic saccharification is the most critical step for recovering fermentable sugars from biomass. Many factors such as pretreatment, enzyme load, pH, temperature, substrate and surfactant concentrations, and reaction time, among others, can affect biomass saccharification. In this sense, different design of experiment (DoE) tools together with response surface methodology (RSM) have been used as a viable strategy to determine how factors influence saccharification and to optimize this process. The strategy involves fewer experiments, less time and lower cost. This review summarizes how DoE and RSM tools have been used to optimize biomass saccharification. Moreover, an overview of the main experimental design tools used in this field is provided.
ISSN:1932-104X
1932-1031
DOI:10.1002/bbb.2534