An Improved Protocol for Rapid in Vitro Multiplication of Clonal Apple Rootstock MM106

The development and homogeneity of apple trees are affected by rootstocks, but the output through conventional methods is seasonal and limited by many factors. The aim of the research was to establish the fastest and most economically viable methodology to facilitate in vitro propagation of clonal a...

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Bibliographic Details
Published in:Erwerbsobstbau 2023-12, Vol.65 (6), p.2241-2248
Main Authors: Lal, Manmohan, Jamwal, Mahital, Bakshi, Parshant, Sharma, Nirmal, Jasrotia, Amit, Sharma, Arti
Format: Article
Language:English
Subjects:
Activated carbon
Activated charcoal
Agriculture
Apples
Biomedical and Life Sciences
Charcoal
Composition
Explants
Fruit trees
Homogeneity
In vitro methods and tests
Life Sciences
Mercuric chloride
Original Article
Originalbeitrag
Plant Sciences
Plantlets
Propagation
Rootstocks
Shoots
Sodium hypochlorite
Survival
Vermiculite
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Summary:The development and homogeneity of apple trees are affected by rootstocks, but the output through conventional methods is seasonal and limited by many factors. The aim of the research was to establish the fastest and most economically viable methodology to facilitate in vitro propagation of clonal apple rootstock MM106. Explants (shoot tip and nodal segment) were surface sterilized using 3.0 to 12.0% NaOCl for 10–30 min and 0.05–0.3% HgCl 2 for 2–6 min. For culture establishment, MS medium was used either alone or with 1.0–3.0 mg/l BAP and 1.0–3.0 mg/l GA 3 . Rooting medium involved half-strength MS medium with 0.5–2.0 mg/litre NAA and 0.5–2.0 mg/l IBA along with 0.2% activated charcoal. The highest culture establishment of 47.77 and 57.77% in the minimum number of days, i.e., 32.30 and 26.33, respectively, while the number of days taken for shoot proliferation (52.40 and 46.26), number of shoots (2.40 and 4.76) and shoot length (3.19 and 3.42 cm), were recorded using the MS + 1.0 mg/l BAP + 1.0 mg/litre GA 3 . The media composition involving half-strength MS + 1.0 mg/litre NAA produced the highest percentage of rooted cultures (60.00) in the fewest number of days amongst all the media compositions. Furthermore, the maximum survival rate (53.33) of in vitro raised plantlets was observed with soil + sand + vermiculite + FYM (1:1:1:1 v/v/v/v) with more leaves.
ISSN:0014-0309
1439-0302
DOI:10.1007/s10341-023-00988-4