Effect of different gas releasing methods on anaerobic fermentative hydrogen production in batch cultures

• Published in: Frontiers of Environmental Science & Engineering 2012-12, Vol.6 (6), p.901-906
• Main Authors: Chang, Sheng, Li, Jianzheng, Liu, Feng, Yu, Ze
• Format: Article
• Language: English
• Subjects: acetates; Acetic acid; Batch culture; batch fermentation; Biogas; biogas releasing; butyrates; Carbon dioxide; Earth and Environmental Science; Environment; Fermentation; Gas streams; glucose; Homoacetogenesis; Hydrogen; hydrogen pressure; Hydrogen production; Mixed culture; Partial pressure; Releasing; Research Article; Sodium hydroxide; Vapor phases; 二氧化碳; 分批培养; 厌氧; 发酵产品; 发酵产氢; 气体释放; 生产系统; 释放气体
• ISSN: 2095-2201; 2095-221X; 1673-7520
• DOI: 10.1007/s11783-012-0403-1

Decreasing hydrogen partial pressure can not only increase the activity of the hydrogen enzyme but also decrease the products inhibition, so it is an appropriate method to enhance the fermentative hydrogen production from anaerobic mixed culture. The effect ofbiogas release method on anaerobic fermentative hydrogen production in batch culture system was compared, i.e., Owen method with intermediately release, continuous releasing method, and continuous releasing ＋ CO2 absorbing. The experi- mental results showed that, at 35℃, initial pH 7.0 and glucose concentration of 10 g.L-1, the hydrogen produc- tion was only 28 mL when releasing gas by Owen method, while it increased two times when releasing the biogas continuously. The cumulative hydrogen production could reach 155 mL when carbon dioxide in the gas stream was continuously absorbed by 1 mol.L-1 NaOH. The results showed that acetate was dominated, accounting for 43% in the dissolved fermentation products in Owen method, whereas the butyrate predominated and reached 47%-53% of the total liquid end products when releasing gas continuously. It is concluded that the homoacetogenesis could be suppressed when absorbing CO2 in the gas phase in fermentative hydrogen production system.