A review of in vitro methods to evaluate the bioaccessibility of phenolic compounds in tropical fruits

International guidelines strongly advise about the frequent and varied intake of plant in diet. In this scenario, the consumption of fruits is closely related to health benefits due to the abundant presence of bioactive substances. Accordingly, the production of tropical fruits has stood out worldwi...

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Bibliographic Details
Published in:Critical reviews in food science and nutrition 2024, Vol.64 (6), p.1780-1790
Main Authors: Gonçalves Santana, Michelle, Freitas-Silva, Otniel, Mariutti, Lilian Regina Barros, Teodoro, Anderson Junger
Format: Article
Language:English
Subjects:
Bioactive compounds
Bioavailability
Cell culture
Dialysis
Digestion
Fermentation
Fruits
In vitro methods and tests
In vivo methods and tests
Phenolic compounds
Phenols
Polyphenols
simulated digestion
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Summary:International guidelines strongly advise about the frequent and varied intake of plant in diet. In this scenario, the consumption of fruits is closely related to health benefits due to the abundant presence of bioactive substances. Accordingly, the production of tropical fruits has stood out worldwide, reaching records since the past decade. However, to ensure that phenolic substances are indeed used by the body, they need to be accessible for absorption. For this purpose, several methods are used to assess the phenomenon of bioaccessibility. We provide information on i) in vitro methods for the evaluation of the bioaccessibility of phenolic compounds in tropical fruits, including their derivatives and by-products; ii) a study performed using a semi-dynamic in vitro digestion model; iii) simulated digestion with a dialysis membrane step, polyphenol transport/uptake using cell culture, and in vitro colonic fermentation process. Although standardized static and semi-dynamic in vitro digestion methods already exist, few studies use these protocols to assess the bioaccessibility of polyphenols in tropical fruits. To guarantee that in vitro digestion assays reproduce consistent results compared to in vivo reference methods, it is essential to universalize standardized methods that allow the comparison between results, enabling the validation of in vitro digestion methods.
ISSN:1040-8398
1549-7852
DOI:10.1080/10408398.2022.2119203