Purple acid phosphatase 10c modifies the rice rhizobacterial community and its phosphorus cycling potential

• Published in: Plant and soil 2024-03, Vol.496 (1-2), p.431-448
• Main Authors: Xing, Hongmei, Luo, Xuesong, Chen, Xinghua, Deng, Suren, Cai, Hongmei, Xu, Fangsen, Shi, Lei, Ding, Guangda, Zhu, Qiang, Wang, Chuang
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Agriculture; Alkaline phosphatase; Bacteria; Biomedical and Life Sciences; Composition; Cycles; Ecological effects; Ecology; Exudates; Exudation; Fertilizers; Life Sciences; Nutrient uptake; Organic phosphorus; Phosphatase; Phosphorus; Plant Physiology; Plant roots; Plant Sciences; Purple acid phosphatase; Relative abundance; Research Article; Rhizosphere; Rice; Rice fields; Soil microorganisms; Soil Science & Conservation
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-023-06374-z

Background and aimes Plant roots secrete acid phosphatases (ACPs) to mineralize rhizosphere organic phosphorus (Po) for absorption. However, the ecological effects of such exudates on the rhizobacterial community and function remain unclear. Methods Purple acid phosphatase 10c (OsPAP10c) is the major root-secreted ACP under both Pi-sufficient and Pi-deficient conditions in rice. To study the effect of root-secreted ACP on rhizosphere phosphorus (P) cycling and succession of the bacterial community, the previously reported OsPAP10c mutant and overexpression lines were planted in paddy soils with or without P fertilizer (+ P or -P). Results The results showed that the expression of OsPAP10c significantly influenced ACP activity in rhizosphere soil, which changed the bacterial composition by 6.72% to 9.54%. The expression of OsPAP10c helped to recruit P-solubilizing bacteria (PSB) at the filling stage under -P conditions. However, OsPAP10c increased bacterial P competition potentials and rhizosphere alkaline phosphatase (ALP) at the elongation and filling stages under + P conditions. Interestingly, overexpression of OsPAP10c relaxed bacterial P demand pressure and increased the relative abundance of bacteria with nitrogen, sulphur, and iron potential functions, which benefited rice nutrient uptake and growth at the early stages. Conclusion In conclusion, this study indicated that P fertilizer and root-secreted ACPs cooperatively regulate rhizosphere P cycling potential by modifying the rhizobacterial composition.