Decellularization techniques of human foreskin for tissue engineering application

The rapid development of tissue engineering (TE) and regenerative medicine brings an acute need for biocompatible and bioactive biological scaffolds to regenerate or restore damaged tissue. Great attention is focused on the decellularization of tissues or even whole organs, and the subsequent coloni...

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Bibliographic Details
Published in:Physiological research 2023, Vol.72 (S3), p.S287-S297
Main Authors: Novotna, O, Varchulova Novakova, Z, Galfiova, P, Lorencova, M, Klein, M, Žiaran, S, Kuniakova, M
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
AIDS
Alcohol
Circumcision
Collagen
Extracellular matrix
Feasibility studies
Glans penis
HIV
Human immunodeficiency virus
Medical research
Penis
Regenerative medicine
Scanning electron microscopy
Tissue engineering
Ultrastructure
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Summary:The rapid development of tissue engineering (TE) and regenerative medicine brings an acute need for biocompatible and bioactive biological scaffolds to regenerate or restore damaged tissue. Great attention is focused on the decellularization of tissues or even whole organs, and the subsequent colonization of such decellularized extracellular matrices by recipient cells. The foreskin is an integral, normal part of the external genitalia that forms the anatomical covering of the glans penis and the urinary meatus of all human and non-human primates. It is mucocutaneous tissue that marks the boundary between mucosa and skin. In this work, we compared two innovative decellularization techniques for human foreskins obtained from donors. We compared the efficacy and feasibility of these protocols and the biosafety of prepared acellular dermal matrixes that can serve as a suitable scaffold for TE. The present study confirms the feasibility of foreskin decellularization based on enzymatic or detergent methods. Both techniques conserved the ultrastructure and composition of natural ECM while being DNA-free and non-toxic, making it an excellent scaffold for follow-up research and TE applications.
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.935185