Genetic insight of recombinant inbred lines of wheat for Karnal bunt resistance using bulk segregant analysis

Karnal bunt (KB) is a seed and airborne wheat disease caused by the fungus Tilletia indica Mitra. The floret-infecting fungus damages grains and adversely affects wheat quality through the production of trimethylamine. Deployment of host-plant resistance is the best strategy to combat this disease....

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Bibliographic Details
Published in:Genetic resources and crop evolution 2024-06, Vol.71 (5), p.1673-1686
Main Authors: Ur Rehman, Attiq, Shabbir, Ghulam, Akram, Zahid, Riaz, Abid
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Bunt
Cultivars
Disease resistance
Fungi
Genomes
Genotyping
Host plants
Inbreeding
Life Sciences
Nucleotides
Plant breeding
Plant Genetics and Genomics
Plant Physiology
Plant resistance
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Polymorphism
Research Article
Single-nucleotide polymorphism
Trimethylamine
Wheat
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Summary:Karnal bunt (KB) is a seed and airborne wheat disease caused by the fungus Tilletia indica Mitra. The floret-infecting fungus damages grains and adversely affects wheat quality through the production of trimethylamine. Deployment of host-plant resistance is the best strategy to combat this disease. The present study explored two sources of genetic resistance to KB by developing recombinant inbred lines (RILs) with a common susceptible parent. Munal × WL711 and SAUAL/KIRITTI/SAUL × WL711 produced 275 RILs each which were evaluated for KB disease resistance under four environments. The minimum value for disease incidence was 0% while the maximum values were 8.14% and 10.38% during 2018–2019 and 2019–2020, respectively. This study identified several RILs with durable and heritable resistance against KB, while SAUAL/KIRITTI/SAUL was identified as the highest-performance resistant parent. Based on the best linear unbiased estimates across environments, two bulk populations (resistant and susceptible) were developed from each set of progeny and were subjected to genome-wide single nucleotide polymorphism (SNP) genotyping using a 50K Affymetrix SNP array. Bulked segregation analysis showed that the maximum SNP index was identified on chr2A, chr6A, and chr7A in Munal × WL-711 while it was on chr4B and chr7B in the SAUAL/KIRITTI/SAUL × WL711 population. Taken together, the identified RILs might serve as breeding material for the development of KB-resistant wheat cultivars in the future.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-023-01731-4