Bioprospecting for Lipid Production of Eleven Microalgae Strains for Sustainable Biofuel Production

• Published in: Bioenergy research 2024-06, Vol.17 (2), p.1118-1132
• Main Authors: Sánchez-Saavedra, M. P., Castro-Ochoa, F. Y.
• Format: Article
• Language: English
• Subjects: Algae; Aquatic microorganisms; Biodiesel fuels; Biofuels; Biomass; Biomedical and Life Sciences; Bioprospecting; Carbohydrates; Cetane number; Composition; Cymbella; Diesel; Dry weight; Fatty acids; Growth rate; Iodine; Life Sciences; Linolenic acid; Lipids; Microalgae; Oleic acid; Palmitic acid; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Productivity; Wood Science & Technology
• ISSN: 1939-1242; 1939-1234; 1939-1242
• DOI: 10.1007/s12155-023-10685-5

The aim of this work was to measure the growth rate, biomass production, proximate composition, and fatty acid content of eleven microalgae strains to evaluate their potential for biofuel production. The growth rate ( p < 0.05), total dry weight ( p < 0.05), organic dry weight ( p < 0.05), ash content ( p < 0.05), and biomass productivity ( p < 0.05) differed among eleven microalgae strains. The proximate composition differed ( p < 0.05) among the eleven microalgae strains. The lipid contents were higher in Cymbella sp. (strain 2) (42.46 ± 1.06%) ( p < 0.05). The carbohydrate content was higher ( p < 0.05) in Aphanocapsa marina (42.40 ± 1.18 %). The protein content was significantly higher ( p < 0.05) in Chlamydomonas mexicana (52.83 ± 0.60%). The contents of saturated (SFAs) ( p < 0.05), monounsaturated (MUFAs), and polyunsaturated (PUFAs) ( p < 0.05) fatty acids among the eleven microalgae strains were different. The most important indicators of biodiesel properties are the cetane number (CN), iodine value (IV), and saponification value (SV). It was concluded that Chlorella vulgaris was an adequate strain to be used in the production of biodiesel due to their high amount of palmitic acid, oleic acid, and alpha-linolenic acid, by high values of IV (147.27 g I 2 /100 g), SV (210.96 mg KOH/g), and CN (48.88). The diatom Cymbella sp. is a promising strain for biodiesel production due to its high values of lipid content (42.46%), lipid productivity (1.24 g/L/day), growth rate (0.67 divisions/day), and IV (197.93 g I 2 /100 g), and by the lowest values of generation time (35.60 h) and SV (208.85 mg KOH/g). Another promising strain for biodiesel production is Porphyridium cruentum due to its high values of biomass productivity (0.038 g/L/day), lipid productivity (0.80 g/L/day), CN (45.60), and IV (220.15 g I 2 /100 g), and by the low generation time and SV (199.25 mg KOH/g).