In Vivo Measurement of Rat Brain Water Content at 9.4T MR Using Super‐Resolution Reconstruction: Validation With Ex Vivo Experiments

BackgroundGiven that changes in brain water content are often correlated with disease, investigating water content non‐invasively and in vivo could lead to a better understanding of the pathogenesis of several neurologic diseases.PurposeTo adapt a super‐resolution‐based technique, previously develop...

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Bibliographic Details
Published in:Journal of magnetic resonance imaging 2024-07, Vol.60 (1), p.161-172
Main Authors: Thomas, Dennis C, Ana‐Maria Oros‐Peusquens, Schöneck, Michael, Willuweit, Antje, Abbas, Zaheer, Zimmermann, Markus, Felder, Jörg, Celik, Avdo, Nadim Joni Shah
Format: Article
Language:English
Subjects:
Animal models
Brain
Brain mapping
Brain stem
Cerebellum
Correlation coefficient
Correlation coefficients
Field strength
Image reconstruction
In vivo methods and tests
Medical imaging
Mesencephalon
Moisture content
Neurological diseases
Pathogenesis
Statistical analysis
Statistical tests
Water
Water content
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Summary:BackgroundGiven that changes in brain water content are often correlated with disease, investigating water content non‐invasively and in vivo could lead to a better understanding of the pathogenesis of several neurologic diseases.PurposeTo adapt a super‐resolution‐based technique, previously developed for humans, to the rat brain and report in vivo high‐resolution (HR) water content maps in comparison with ex vivo wet/dry methods.Study TypeProspective.Animal ModelEight healthy male Wistar rats.Field Strength/Sequence9.4‐T, multi‐echo gradient‐echo (mGRE) sequence.AssessmentUsing super‐resolution reconstruction (SRR), a HR mGRE image (200 μm isotropic) was reconstructed from three low‐resolution (LR) orthogonal whole‐brain images in each animal, which was followed by water content mapping in vivo. The animals were subsequently sacrificed, the brains excised and divided into five regions (front left, front right, middle left, middle right, and cerebellum–brainstem regions), and the water content was measured ex vivo using wet/dry measurements as the reference standard. The water content values of the in vivo and ex vivo methods were then compared for the whole brain and also for the different regions separately.Statistical TestsFriedman's non‐parametric test was used to test difference between the five regions, and Pearson's correlation coefficient was used for correlation between in vivo and ex vivo measurements. A P‐value
ISSN:1053-1807
1522-2586
DOI:10.1002/jmri.29061