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ArdA Protein Specificity against Type I Restriction–Modification Systems
The ArdA DNA-mimic antirestriction proteins inhibit type I restriction−modification (RMI) systems by binding instead of DNA to RMI. The ArdA specificity to DNA methylation sites recognized by RMI complexes remains poorly understood; i.e., it is unclear whether a particular DNA site is mimicked by Ar...
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Published in: | Molecular biology (New York) 2024, Vol.58 (3), p.527-533 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | The ArdA DNA-mimic antirestriction proteins inhibit type I restriction−modification (RMI) systems by binding instead of DNA to RMI. The ArdA specificity to DNA methylation sites recognized by RMI complexes remains poorly understood; i.e., it is unclear whether a particular DNA site is mimicked by ArdA. The
ardA
genes were cloned from three Gram-positive bacteria:
Agrobacterium tumefaciens
,
Pseudomonas monteilii,
and
Xanthomonas
sp
.
Antirestriction activities of their products were tested against three
Escherichia coli
RMI systems differing in DNA recognition/methylation sites. Although similar structures were predicted for the ArdA proteins, the strong specificity to three RMI systems was observed. The results indicate that specific DNA sites may be imitated by DNA mimic ArdA proteins. |
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ISSN: | 0026-8933 1608-3245 |
DOI: | 10.1134/S0026893324700158 |