Development of Analytical method by UV and HPLC of Acetazolamide in bulk and tablet dosage form

In present study, on a Jasco V-730 spectrophotometer, the method for estimating acetazolamide was used, with ethanol: distilled water (5:5) serving as the solvent. For acetazolamide, the recovery analysis research indicates recoveries of 102.55%, 102.68%, and 103.35% for various concentrations of th...

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Bibliographic Details
Published in:Asian Journal of Pharmaceutical Analysis 2024-06, Vol.14 (2), p.60-64
Main Author: Chougule, S. P.
Format: Article
Language:English
Subjects:
Calibration
Drug dosages
Ethanol
Pharmaceutical industry
Solvents
Water
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Summary:In present study, on a Jasco V-730 spectrophotometer, the method for estimating acetazolamide was used, with ethanol: distilled water (5:5) serving as the solvent. For acetazolamide, the recovery analysis research indicates recoveries of 102.55%, 102.68%, and 103.35% for various concentrations of the spiked medication. For quantifying acetazolamide in bulk and tablet dosage form, an RP-HPLC method was created and validated with mobile phase acetonitrile: water (80:20). For acetazolamide, the assay (% w/w) was found 98.6 %, 98.5 %, 98.9 %. The flow rate was 1ml/min. The sample volume was 20.00µl. Run time 5 min and retention time was 2.044.
ISSN:2231-5667
2231-5675
DOI:10.52711/2231-5675.2024.00011