Reuse of Grape Pomace and Wheat Bran for Biosynthesis of On-site Lignocellulose-degrading Enzymes by Trametes villosa and Trichoderma asperellum Under Solid State Fermentation

In this study, the biosynthesis of lignocellulose-degrading enzymes by Trametes villosa and Trichoderma asperellum , in monocultures and in consortium, was evaluated via solid state fermentation (SSF) using wheat bran (WB) and grape pomace (GP) as carbon sources. Among the investigated enzymes, ß-gl...

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Bibliographic Details
Published in:Waste and biomass valorization 2024, Vol.15 (8), p.4747-4760
Main Authors: Corrêa, Anderson Gabriel, de Oliveira Rodrigues, Patrísia, de Azevedo, Lucas Carvalho Basilio, Pasquini, Daniel, Baffi, Milla Alves
Format: Article
Language:English
Subjects:
Biorefineries
Biosynthesis
Carbon sources
Consortia
Engineering
Environment
Environmental Engineering/Biotechnology
Enzymes
Fermentation
Fungi
Glucosidase
Grapes
Hemicellulases
Industrial Pollution Prevention
Lignocellulose
Monoculture
Original Paper
Renewable and Green Energy
Solid state
Solid state fermentation
Substrates
Thermal stability
Trametes
Trichoderma asperellum
Waste Management/Waste Technology
Wheat
Wheat bran
Xylanase
Xylosidase
β-Glucosidase
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Summary:In this study, the biosynthesis of lignocellulose-degrading enzymes by Trametes villosa and Trichoderma asperellum , in monocultures and in consortium, was evaluated via solid state fermentation (SSF) using wheat bran (WB) and grape pomace (GP) as carbon sources. Among the investigated enzymes, ß-glucosidase exhibited the highest production (218.91 U/gds) (units per gram of dry substrate) in SSF carried out with T. villosa and WB as substrate (F4). The peaks of hemicellulases (19.39 U/gds for ß-xylosidase and 170.28 U/gds for xylanase) were produced in SSF performed with fungal consortium and WB. The maximum laccase biosynthesis (16.5 U/gds) was obtained by T. villosa cultured in the mixed GP and WB substrates. The β-glucosidase from T. villosa (F4) showed optimal pH and temperature at 4.5 and 65 °C, as well as pH stability at 4.5–5.5 and thermostability at 45–65 °C, demonstrating acidophilic and thermophilic properties which are suitable for future application in saccharifications. The findings provided the efficient management of these agro-industrial by-products for the enzyme production with potential use in biorefinery. Graphical Abstract
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-024-02502-7