Fermentation process optimization by response surface methodology for bioethanol production from argane pulp hydrolysate using commercial and laboratory scale isolated Saccharomyces cerevisiae yeast

• Published in: Biomass conversion and biorefinery 2024-08, Vol.14 (15), p.16891-16898
• Main Authors: Zouhair, Fatima Zahrae, Kabbour, Mohammed Rachid, Moussaid, Siham, Ebich, Fatima, Bouksaim, Mohammed, Lgaz, Hassane, Cho, Youngjae, Essamri, Azzouz
• Format: Article
• Language: English
• Subjects: Biofuels; Biotechnology; Energy; Fermentation; Hydrolysates; Liquid fuels; Optimization; Original Article; Productivity; Renewable and Green Energy; Response surface methodology; Substrates; Yeast
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-023-03836-3

In recent decades, growing attention was devoted to reducing fossil-fuel usage. The production of bioethanol from natural sources is an eco-friendly liquid fuel alternative that can be used in a wide range of applications. In this work, Argane ( Argania spinosa (L.) Skeels ) pulp hydrolysate was used, for the first time, as a substrate for bioethanol production, aiming to improve the valorization of this local biowaste as a new biomass for biofuel production. Hence, a comparative fermentation study of argane pulp hydrolysate was investigated using commercial and laboratory-scale isolated Saccharomyces cerevisiae Meyen ex EC Hansen yeast ( S. cerevisiae ). The experiments of the alcoholic fermentation were conducted under the influence of temperature, pH, and yeast concentration on bioethanol yield and sugar consumption using response surface methodology. A maximum bioethanol yield of 5.91 mg/mL was observed under optimal process conditions of 32.5 °C (temperature), 5.5 (pH), and 4.50% of isolated S. cerevisiae . The results showed that isolated S. cerevisiae ( Saxapahaw-DS1693 ) yeast, with a highest yield of 5.91 mg/mL and productivity of 0.098 g/Lh, was more efficient than the commercial S. cerevisiae , with a highest yield of 2.43 mg/mL and productivity of 0.040 g/Lh, during 60 h. Optimization and findings obtained from this study would provide significant knowledge for bioethanol production processes using argane pulp hydrolysate as a new and low-cost option to mitigate the local energy demand.