Determination of allele frequencies in 15 autosomal short tandem repeat (STR) loci of Arab residents in Iraq

Recent advances in genetic research and molecular techniques have significantly contributed to our understanding of human population genetics and its diverse applications. One such area of investigation is the study of Short Tandem Repeats (STRs). By analyzing representative samples from different A...

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Bibliographic Details
Published in:Nucleus (Calcutta) 2024-08, Vol.67 (2), p.447-453
Main Authors: Al-Zubaidi, Mohammed Mahdi, Al-Rubai, Haider Khadum, Khadum, Sara Talb
Format: Article
Language:English
Subjects:
Alleles
Biomedical and Life Sciences
Cell Biology
Gene frequency
Genetic diversity
Genotypes
Life Sciences
Population genetics
Population studies
Research Report
Short tandem repeats
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Summary:Recent advances in genetic research and molecular techniques have significantly contributed to our understanding of human population genetics and its diverse applications. One such area of investigation is the study of Short Tandem Repeats (STRs). By analyzing representative samples from different Arab groups, the researchers sought to determine allele frequencies at 15 specific STR loci using PCR amplification and fragment analysis. The study involved analyzing a sample of 106 individuals from Arab residing in Iraq using the AmpFlSTR1® Identifiler™ PCR Amplification Kit from the Applied Biosystem to evaluate 15 STR, including D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, CSF1PO, D19S433, D2S1338, and D16S539. It is found that the 15 short tandem repeat (STR) loci analyzed in the population displayed different numbers of alleles with frequencies ranging from 0.005 to 0.443. The analysis also revealed that the population was in Hardy–Weinberg equilibrium, indicating that the observed genotype frequencies were consistent with the expected frequencies under the assumptions of the equilibrium. Moreover, the study showed high combined power of discrimination (CPE) and power of exclusion (P.E.) values for all 15 STR loci, with values of 0.9998 and 0.9999, respectively. Additionally, the probability of matching value was calculated to be 1.059, indicating that the population exhibited high genetic diversity.
ISSN:0029-568X
0976-7975
DOI:10.1007/s13237-023-00442-6