RP-HPLC Method Development and Validation for Simultaneous Estimation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form

Objective: A reverse- phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for estimation of combined formulation containing tenofovir(disoproxilfumarate) (TDF) and emtricitabine (EMT). Method: Chromatographic separation was performed on RP-C18 column. The optim...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2024-05, Vol.17 (5), p.2229-2234
Main Authors: Lakade, Mayuri M, Kale, RN, Dyade, GK
Format: Article
Language:English
Subjects:
Accuracy
Acids
Calibration
Chromatography
Drug dosages
HIV
Human immunodeficiency virus
Molecular weight
Pharmaceuticals
Solvents
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Summary:Objective: A reverse- phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for estimation of combined formulation containing tenofovir(disoproxilfumarate) (TDF) and emtricitabine (EMT). Method: Chromatographic separation was performed on RP-C18 column. The optimized mobile phase of composition (Methanol: Water and pH adjusted to 3.8 with acetic acid was pumped withflow rate of 0.8ml/min in the 70:30% v/v) ratio and the eluents were monitored at 261nm. Results: The assay was performed with tablet and percentage of assay was found to 99.91% for TDF and 99.69% for EMT respectively. Linearity was obtained in the concentration range of 2-12µg/ml for TDF and 2-12µg/ml for EMT. The method was statistically validated and RSD was found to be
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.52711/0974-360X.2024.00351