Functional and structural neurodegenerative activities of Ankaferd BloodStopper in a mouse sciatic nerve model

Traumatic and postoperative hemorrhages are life-threatening complications. Ankaferd BloodStopper (ABS) is a potent topical hemostatic agent to stop bleeding. However, ABS is associated with nerve toxicity. The present study aimed to investigate the functional and structural neurodegenerative effect...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2024-09, Vol.28 (3), Article 345
Main Authors: Ustun, Ramazan, Oguz, Elif Kaval, Seker, Ayse, Taspinar, Filiz
Format: Article
Language:English
Subjects:
Anticoagulants
Care and treatment
Clinical medicine
Dentistry
Diagnosis
Gait
Gait disorders
Health aspects
Immunohistochemistry
Ketamine
Laboratory animals
Nervous system
Sciatic nerve
Surgery
Toxicity
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Summary:Traumatic and postoperative hemorrhages are life-threatening complications. Ankaferd BloodStopper (ABS) is a potent topical hemostatic agent to stop bleeding. However, ABS is associated with nerve toxicity. The present study aimed to investigate the functional and structural neurodegenerative effects of ABS in a mouse model. A total of 30 male BALB/c mice, aged 6-8 weeks, were randomly divided into control group (no treatment), a sham group (treated with saline) and an experimental group (treated with ABS). In the saline and the ABS groups, the right sciatic nerve was surgically exposed and treated with saline or ABS, respectively. No surgical procedure was performed in the control group. On day 7 post-treatment, functional changes of the sciatic nerve were evaluated by a horizontal ladder rung walking task. Structural changes were assessed with immunohistochemistry. In the horizontal ladder rung walking test, the gait impairment was proportional to the severity of sciatic nerve damage, with the ABS group showing a significantly higher rate of errors than the control and saline groups. Immunohistochemistry demonstrated extensive degeneration and deformation in the axons and myelin sheath of the sciatic nerve in the ABS group. The results provide compelling evidence for the neurotoxicity of ABS.
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2024.12634