New validated uv spectrophotometric methods for the determination of sparfloxacin in tablets

Two new, simple, economical, sensitive UV spectrophotometric methods (fundamental method (A) and area under curve (B)) have been developed for the quantitative estimation of sparfloxacin in bulk drug and pharmaceutical dosage forms. Sparfloxacin was estimated at 290 nm in phosphate buffer 6.8 and bo...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2020-08, Vol.13 (8), p.3587-3592
Main Authors: Sowjanya, G., Sirisha, Ch, Prasad, M. Krishna
Format: Article
Language:English
Subjects:
Accuracy
Calibration
Drug dosages
Methods
Regression analysis
Solvents
Spectrum analysis
Online Access:Get full text
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Summary:Two new, simple, economical, sensitive UV spectrophotometric methods (fundamental method (A) and area under curve (B)) have been developed for the quantitative estimation of sparfloxacin in bulk drug and pharmaceutical dosage forms. Sparfloxacin was estimated at 290 nm in phosphate buffer 6.8 and borate buffer 9.0. In method A and method B, the drug obeyed linearity in the range of 1-50μg/mL for both the buffers. The methods were tested and validated for various parameters according to ICH guidelines. The % RSD obtained in intraday, interday precision and robustness studies were found to be < 1.3 and the recovery of the drug was found to be 98.2 – 101.6% indicating the accuracy of the methods. The methods also proved to be sensitive as observed from the LOD and LOQ values. The proposed methods were successfully applied for the determination of sparfloxacin in tablets without any interference from excipients.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2020.00634.4