High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system

Cellular mechanical properties influence cellular functions across pathological and physiological systems. The observation of these mechanical properties is limited in part by methods with a low throughput of acquisition or with low accessibility. To overcome these limitations, we have designed, dev...

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Bibliographic Details
Published in:Microfluidics and nanofluidics 2024-12, Vol.28 (12), p.78, Article 78
Main Authors: Smith, Ian M., Ursitti, Jeanine A., Majeti Venkata, Sai Pranav, Givpoor, Nikka, Stemberger, Megan B., Hengen, Autumn, Banerjee, Shohini, Hached, Khaled, van der Laan, Siem, Stains, Joseph, Martin, Stuart S., Ward, Christopher, Stroka, Kimberly M.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biomedical Engineering and Bioengineering
Cell culture
Cytoskeleton
Deformation
Drug screening
Engineering
Engineering Fluid Dynamics
Kinases
Mechanical properties
Medical research
Medicine
Microfluidic devices
Microfluidics
Nanotechnology and Microengineering
Pathology
Velocity
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Summary:Cellular mechanical properties influence cellular functions across pathological and physiological systems. The observation of these mechanical properties is limited in part by methods with a low throughput of acquisition or with low accessibility. To overcome these limitations, we have designed, developed, validated, and optimized a microfluidic cellular deformation system (MCDS) capable of mechanotyping suspended cells on a population level at a high throughput rate of ~ 300 cells per second. The MCDS provides researchers with a viable method for efficiently quantifying cellular mechanical properties towards defining prognostic implications of mechanical changes in pathology or screening drugs to modulate cytoskeletal integrity.
ISSN:1613-4982
1613-4990
DOI:10.1007/s10404-024-02774-4