Metabolic Engineering of Komagataella phaffii for Xylose Utilization from Cellulosic Biomass

Cellulosic biomass hydrolysates are rich in glucose and xylose, but most microorganisms, including Komagataella phaffii, are unable to utilize xylose effectively. To address this limitation, we engineered a K. phaffii strain optimized for xylose metabolism through the xylose oxidoreductase pathway a...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2024-01, Vol.29 (23), p.5695
Main Authors: Park, Jongbeom, Park, Sujeong, Grace Evelina, Kim, Sunghee, Yong-Su, Jin, Won-Jae, Chi, In Jung Kim, Kim, Soo Rin
Format: Article
Language:English
Subjects:
Amino acids
Biomass
Carbon
Cellulose
Consumption
Crude oil prices
Enzymes
Fermentation
Gene expression
Glucose
Libraries
Metabolism
Metabolites
Nitrogen
Proteins
Yeast
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Summary:Cellulosic biomass hydrolysates are rich in glucose and xylose, but most microorganisms, including Komagataella phaffii, are unable to utilize xylose effectively. To address this limitation, we engineered a K. phaffii strain optimized for xylose metabolism through the xylose oxidoreductase pathway and promoter optimization. A promoter library with varying strengths was used to fine-tune the expression levels of the XYL1, XYL2, and XYL3 genes, resulting in a strain with a strong promoter for XYL2 and weaker promoters for XYL1 and XYL3. This engineered strain exhibited superior growth, achieving 14 g cells/L and a maximal growth rate of 0.4 g cells/L-h in kenaf hydrolysate, outperforming a native strain by 17%. This study is the first to report the introduction of the xylose oxidoreductase pathway into K. phaffii, demonstrating its potential as an industrial platform for producing yeast protein and other products from cellulosic biomass.
ISSN:1420-3049
DOI:10.3390/molecules29235695