Untangling Heparan Sulfate 3‑O‑Sulfation Using a Novel Offline Cationic-Peptide Affinity Enrichment, Followed by HILIC-cIM-MS

Heparan sulfate (HS) is a linear polysaccharide that modifies proteoglycans. HS biosynthesis is regulated in a spatiotemporal manner, leading to structural diversity, including variable de-N-acetylation, N-sulfation, hexuronic acid C5 epimerization, and 2-O-, 6-O-, and 3-O-sulfation. Specific struct...

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Bibliographic Details
Published in:	Analytical chemistry (Washington) 2025-04, Vol.97 (16), p.8700-8708
Main Authors:	Mernie, Elias, Cavallero, Gustavo J., Xia, Chaoshuang, Lin, Cheng, Zaia, Joseph
Format:	Article
Language:	English
Subjects:	Acetylation Affinity analytical chemistry Animals Antithrombin Binding sites Biosynthesis Carbohydrates Cations Cations - chemistry Chromatography, Liquid Enrichment Heparan sulfate Heparin Heparin lyase Heparitin Sulfate - analysis Heparitin Sulfate - chemistry Heparitin Sulfate - metabolism hydrophilicity Hydrophobic and Hydrophilic Interactions intestinal mucosa Ionic mobility Liquid chromatography Mass spectrometry Mass Spectrometry - methods Mass spectroscopy Neurological diseases Oligosaccharides Peptides Peptides - chemistry Polysaccharides Proteoglycans Scientific imaging Selective binding Sulfates Sulfates - chemistry Sulfation swine Tandem Mass Spectrometry
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