Low‐Background His‐Tag‐Targeting Probes for Turn‐On Fluorescence Detection of Cell Surface Proteins and Their Binding Interactions

Turn‐on fluorescent probes consisting of dye‐ligand conjugates serve as a powerful tool for detecting cell surface proteins (CSPs) and their interactions with binding partners. However, generating such probes from protein‐based ligands remains challenging. This challenge became particularly evident...

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Published in:	Small (Weinheim an der Bergstrasse, Germany) Germany), 2025-08, Vol.21 (33), p.e2411730-n/a
Main Authors:	Prasad, Pragati Kishore, Toraskar, Suraj, Khan, Suman, Granot, Tom, Sirkis, Yael Fridmann, Yardeni, Eliane Hadas, Albeck, Shira, Unger, Tamar, Petrovich‐Kopitman, Ekaterina, Addadi, Yoseph, Raigawali, Rakesh, Anand, Saurabh, Vishweshwara, Sharath S., Shanthamurthy, Chethan D., Oppenheimer‐Low, Noa, Kikkeri, Raghavendra, Avinoam, Ori, Motiei, Leila, Margulies, David
Format:	Article
Language:	English
Subjects:	Angiotensin-Converting Enzyme 2 - metabolism Binding COVID-19 - virology Dyes Emission spectra Fluorescence Fluorescent Dyes - chemistry Fluorescent indicators Histidine - chemistry Histidine - metabolism His‐tag binding probe Humans Ligands Membrane Proteins - metabolism Nitrilotriacetic acid Nitrilotriacetic Acid - chemistry Protein Binding protein surface recognition Proteins Quinoline Receptors receptor‐ligand interaction SARS-CoV-2 - metabolism Spike Glycoprotein, Coronavirus - chemistry Spike Glycoprotein, Coronavirus - metabolism thiazole orange turn‐on fluorescent probes Viral diseases
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