Efficient Biosynthesis of Polyhydroxybutyrate from Xylose via an In Vitro Synthetic Enzymatic Biosystem with Self‐Sustained Cofactor Regeneration

In vitro biotransformation mediated by in vitro synthetic enzymatic biosystems (ivSEBs) represents a highly promising platform for sustainable biomanufacturing, offering enhanced reaction efficiency by circumventing cellular constraints. This study develops an ivSEB comprising 17 enzymes for the cel...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2025-09, Vol.26 (16), p.e202500387-n/a
Main Authors: Qiu, Boyu, Wei, Xinlei, You, Chun
Format: Article
Language:English
Subjects:
Acetyl Coenzyme A - metabolism
Adenosine diphosphate
Biosynthesis
Biotransformation
Coenzyme A
Coenzymes - metabolism
Cofactors
enzyme cascades
Enzymes
Glycolysis
Hydroxybutyrates - chemistry
Hydroxybutyrates - metabolism
in  vitro synthetic enzymatic biosystems
Pentose
Pentose phosphate pathway
Polyesters - chemistry
Polyesters - metabolism
Polyhydroxybutyrate
Polyhydroxybutyrates
Polyhydroxybutyric acid
Xylose
Xylose - chemistry
Xylose - metabolism
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