total polyphenols and the antioxidant potentials of some selected cereals and pseudocereals

• Published in: European food research & technology 2007-07, Vol.225 (3-4), p.321-328
• Main Authors: Gorinstein, Shela, Vargas, Oscar J. Medina, Jaramillo, Nicolas O, Salas, Ines Arnao, Ayala, Alma Leticia Martinez, Arancibia-Avila, Patricia, Toledo, Fernando, Katrich, Elena, Trakhtenberg, Simon
• Format: Article
• Language: English
• Subjects: Antioxidants; Biological and medical sciences; Bleaching; Cereal and baking product industries; Cereals; Correlation coefficient; Dry matter; Flavonoids; Food industries; Fundamental and applied biological sciences. Psychology; Methanol; Nutrition; Peroxidation; Phenols; Polyphenols; Pseudocereals; Quinoa; seeds; Selected cereals; Soybeans
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-006-0417-7

The objective of the present study was to investigate the effect of phenolic substances and proteins on the antioxidant potentials in some cereals and pseudocereals and to compare their bioability. The polyphenol dry matter extracts (PDME) from the investigated seeds of buckwheat, rice, soybean, amaranth and quinoa with 1.2 M HCl in 50% methanol/water (PDME50%Met/HCl) exhibited higher inhibition of lipid peroxidation than the ones extracted with 50% methanol/water (PDME50%Met) and were comparable to the antioxidant activity of butylated hydroxyanisole at concentration of 0.2 mg mL-¹. The antioxidant activities of these seed extracts determined by 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate)-ABTS⁺/K₂S₂O₈, β-carotene bleaching (β-carotene), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging methods showed high correlation coefficients (R ²) such as 0.9515, 0.9058 and 0.8723, respectively, with the presence of total polyphenols estimated by Folin-Ciocalteu assay. These results indicate that the major antioxidant components in these extracts mostly derived from the polyphenols, and proteins showed only minimal values of bioactivity. Based on high contents of polyphenols, anthocyanins, flavonoids and their antioxidant activities pseudocereals such as buckwheat, quinoa and amaranth can be a substitute for cereals for common and atherosclerotic diets and sometimes in the allergic cases.