Preliminary investigations on a new disposable potentiometric biosensor for uric acid

In this paper, uricase, catalase, and electron mediator were coimmobilized on the surface of the tin oxide (SnO/sub 2/)/indium tin oxide (ITO) glass, to develop a disposable potentiometric uric acid biosensor. The SnO/sub 2/ / ITO glass was employed as a pH sensor, fabricated by sputtering SnO/sub 2...

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Bibliographic Details
Published in:IEEE transactions on biomedical engineering 2006-07, Vol.53 (7), p.1401-1408
Main Authors: Liao, Cheng Wei, Chou, Jung Chuan, Sun, Tai Ping, Hsiung, Shen Kan, Hsieh, Jui Hsiang
Format: Article
Language:English
Subjects:
3-glycidyloxypropyltrimethoxysilane (GPTS)
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Electrochemistry - instrumentation
Electrochemistry - methods
Electron mediator
Electrons
Equipment Design
Equipment Failure Analysis
Glass
Hydrogen-Ion Concentration
Indium tin oxide
Indium tin oxides
Interference
Microelectrodes
pH sensor
Pilot Projects
Reproducibility of Results
Sensitivity and Specificity
Sputtering
Studies
Sugar
Testing
Thin film sensors
Urate Oxidase - analysis
Urate Oxidase - chemistry
Uric Acid - urine
uricase
Urinalysis - instrumentation
Urinalysis - methods
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Summary:In this paper, uricase, catalase, and electron mediator were coimmobilized on the surface of the tin oxide (SnO/sub 2/)/indium tin oxide (ITO) glass, to develop a disposable potentiometric uric acid biosensor. The SnO/sub 2/ / ITO glass was employed as a pH sensor, fabricated by sputtering SnO/sub 2/ thin films on the ITO glass. 3-Glycidyloxypropyltrimethoxysilane (GPTS) was utilized to immobilize uricase, catalase and the electron mediator (ferrocenecarboxylic acid, FcA) on the sensing window. The experimental results reveal that the optimal weight ratio of uricase, FcA to catalase (CAT) is 4:1:2. The sensor responds linearly between 2 mg/dl and 7 mg/dl at pH 7.5, in 20 mM of test solution, with a correlation coefficient of 0.99213. Accordingly, no significant interference was observed when interfering substances, glucose, urea and ascorbic acid, were added to the uric acid solution. Moreover, the recorded voltage was relatively constant during the first 28 days of measurement. Consequently, a potentiometric uric acid biosensor was realized with the advantages of low cost and simple fabrication.
ISSN:0018-9294
1558-2531
DOI:10.1109/TBME.2006.875720