Antibody Immobilization on Conductive Polymer Coated Nonwoven Fibers for Biosensors

This work is being performed to develop rapid and novel electrochemical biosensors for foodborne pathogen detection. This research focuses on electrotextile platforms to perform both capture and sensing functions in a single component. The biosensor uses nonwoven fiber membranes coated with conducti...

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Bibliographic Details
Published in:Sensors & transducers 2011-12, Vol.13, p.142
Main Authors: Mcgraw, Shannon K, Anderson, Michael J, Alocilja, Evangelyn C, Marek, Patrick J, Senecal, Kris J, Senecal, Andre G
Format: Article
Language:English
Subjects:
Adsorption
Binding sites
E coli
Electrodes
Food safety
Membranes
Methods
Signal transduction
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Summary:This work is being performed to develop rapid and novel electrochemical biosensors for foodborne pathogen detection. This research focuses on electrotextile platforms to perform both capture and sensing functions in a single component. The biosensor uses nonwoven fiber membranes coated with conductive polymer and functionalized with antibodies for biological capture. This study examines three methods for antibody immobilization: passive adsorption, glutaraldehyde cross-linking, and EDC/Sulfo-NHS cross-linking. Antibodies are immobilized onto the conductive fiber surfaces for the specific capture of a target pathogen. The immobilization and capture capabilities of each method are analyzed through the use of two different fluorescent reporters: FITC and PicoGreen DNA stain. Fluorescence is measured using a fluorescent plate reader and then imaged using a fluorescent microscope. The effect of a blocking agent on specificity is also evaluated. It is found that glutaraldehyde with blocking is the best immobilization method with PicoGreen being the best fluorescent reporter. [PUBLICATION ABSTRACT]
ISSN:2306-8515
1726-5479