Sugarcane metabolites produced in CO₂-rich temporary immersion bioreactors (TIBs) induce tomato (Solanum lycopersicum) resistance against bacterial wilt (Ralstonia solanacearum)

The production of phenolic metabolites has been optimized in parallel to sugarcane micropropagation in temporary immersion bioreactors (TIBs). Culturing micropropagated plants in 0.4% CO₂-rich air induced their photosynthetic activity by enhancing the change from a heterotrophic to a photomixotrophi...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2010-12, Vol.46 (6), p.558-568
Main Authors: Yang, Liu, Zambrano, Yumarys, Hu, Chun-Jin, Carmona, Elva R, Bernal, Aydiloide, Pérez, Alicia, Zayas, Carlos M, Li, Yang-Rui, Guerra, Abby, Santana, Ignacio, Arencibia, Ariel D
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Bioreactors
Carbon dioxide
Cell Biology
Culture media
Developmental Biology
Genetic engineering
Inoculation
Life Sciences
Metabolic diseases
Metabolites
Micropropagation
Pathogens
Phenols
PHYSIOLOGY
Plant Breeding/Biotechnology
Plant diseases
Plant Genetics and Genomics
Plant Sciences
Plant Tissue Culture
Plants
Seeds
Sugar cane
Sugarcane
Tomatoes
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Summary:The production of phenolic metabolites has been optimized in parallel to sugarcane micropropagation in temporary immersion bioreactors (TIBs). Culturing micropropagated plants in 0.4% CO₂-rich air induced their photosynthetic activity by enhancing the change from a heterotrophic to a photomixotrophic metabolic stage. Under 0.4% CO₂ enrichment, the transcript levels of both phenylalanine ammonia-lyase (PAL EF189195) and ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco CF576750) increased and were correlated to a 4.6- and 6.3-fold increase in the phenolic levels when plants were multiplied in 20 or 30 g/l sucrose, respectively. A novel application of plant phenolic metabolites as elicitors of resistance to tomato bacterial wilt in the Solanum lycopersicum-Ralstonia solanacearum pathosystem has been identified. The culture media was collected, and the phenolics were sprayed onto tomato plants infected with R. solanacearum, eliciting and/or maintaining an early defense signaling mechanism that resulted in the protection of the plant against the tomato bacterial wilt disease. RT-PCR analyses confirmed that selected genes from defense-related pathways were differentially expressed between plants treated with sugarcane metabolites, non-treated pathogen-free plants, and non-treated plants inoculated with R. solanacearum.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-010-9312-9