Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices

Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined l...

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Bibliographic Details
Published in:Analytical biochemistry 2012-05, Vol.424 (2), p.195-205
Main Authors: Braddick, Lucy M., Garland, Patrick J., Praeger, Matthew F., Butement, Jonathan, Friedrich, Daniel, Morgan, David J., Melvin, Tracy
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Avidin - chemistry
Bioaffinity sensors
Bioconjugation
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotin - chemistry
Carbocyanines
Cytokines - blood
denaturation
Dextrans - chemistry
Humans
integration
Lab-on-a-chip
Lab-On-A-Chip Devices
Microcontact printing
Microfabrication
Microfluidic
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microtechnology
Photoelectron Spectroscopy
proteins
reproduction
silicon
Silicon Dioxide - chemistry
Tumor Necrosis Factor-alpha - chemistry
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Summary:Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-“inked” stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2012.02.020