Characterization of a carcinogenesis-associated long non-coding RNA

• Published in: RNA biology 2012-01, Vol.9 (1), p.110-116
• Main Authors: Yang, Feng, Yi, Fan, Zheng, Zhiguo, Ling, Zhiqiang, Ding, Jianv, Guo, Jiangfeng, Mao, Weimin, Wang, Xiongbiao, Wang, Xiaoxia, Ding, Xianfeng, Liang, Zicai, Du, Quan
• Format: Article
• Language: English
• Subjects: Binding; Biology; Biomarkers, Tumor - analysis; Biomarkers, Tumor - genetics; Bioscience; Calcium; Cancer; Case-Control Studies; Cell; Cell Cycle; Chromosomes, Human, Pair 1 - genetics; Cloning, Molecular; Cycle; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Genetic Vectors; Genome, Human; HEK293 Cells; HeLa Cells; Humans; Landes; long non-coding RNA; Neoplasms - genetics; Organogenesis; Proteins; RNA, Messenger - analysis; RNA, Messenger - genetics; RNA, Neoplasm - genetics; RNA, Untranslated - genetics; Transfection; tumor
• ISSN: 1547-6286; 1555-8584
• DOI: 10.4161/rna.9.1.18332

A negative selection strategy was used in the present study to isolate long polyA-minus RNAs from the total transcriptome and a long non-coding RNA named Yiya was identified. Yiya is a 1.9 kb long intergenic ncRNA gene mapped to chromosome 1q41, a well-established cancer susceptibility locus. Expression profiling revealed a general and regulated expression pattern of Yiya in major tissues, and more interestingly, identified elevated mRNA levels in different cancers. Quantitative analysis further demonstrated a dynamic regulation of Yiya expression in cell cycle progression, suggesting that it was involved in cell cycle regulation. Supporting this, overexpression of Yiya promotes cell cycle progression at the G1/S transition, therefore identifying Yiya as a cell-cycle-associated long non-coding RNA.