Immunolocalization of nestin, mesothelin and epithelial membrane antigen (EMA) in developing and adult serous membranes and mesotheliomas

The spatial and temporal distribution of epithelial membrane antigen (EMA), mesothelin and nestin was immunohistochemically analyzed in developing and adult human serous membranes and mesotheliomas in order to detect possible differences in the course of mesenchymal to epithelial transformation, whi...

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Published in:Acta histochemica 2012-09, Vol.114 (5), p.469-479
Main Authors: Petricevic, Josko, Punda, Hrvoje, Brakus, Snjezana Mardesic, Vukojevic, Katarina, Govorko, Danijela Kalibovic, Alfirevic, Darko, Kvesic, Ante, Saraga-Babic, Mirna
Format: Article
Language:English
Subjects:
Aged
Epithelial membrane antigen
Female
GPI-Linked Proteins - analysis
Human development
Humans
Immunohistochemistry
Intermediate Filament Proteins - analysis
Male
Mesenchyme-epithelial transformation
Mesothelin
Mesothelioma - metabolism
Mesothelioma - pathology
Mesotheliomas
Middle Aged
Mucin-1 - analysis
Nerve Tissue Proteins - analysis
Nestin
Serous Membrane - embryology
Serous Membrane - metabolism
Serous membranes
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Summary:The spatial and temporal distribution of epithelial membrane antigen (EMA), mesothelin and nestin was immunohistochemically analyzed in developing and adult human serous membranes and mesotheliomas in order to detect possible differences in the course of mesenchymal to epithelial transformation, which is associated with differentiation of mesothelial cells during normal development and tumorigenesis. Pleura and pericardium developing from the visceral mesoderm gradually transform into mesothelial cells and connective tissue. EMA appeared in mesothelium of both serous membranes during the early fetal period, whereas during further development, EMA expression was retained only in the pericardial mesothelium. It increased in both pleural mesothelium and connective tissue. Mesothelin appeared first in pericardial submesothelial cells and later in surface mesothelium, while in pleura it was immediately localized in mesothelium. In adult serous membranes, EMA and mesothelin were predominantly expressed in mesothelium. Nestin never appeared in mesothelium, but in connective tissues and myocardial cells and subsequently decreased during development, apart from in the walls of blood vessels. Mesothelial cells in the two serous membranes developed in two separate developmental pathways. We speculate that submesothelial pericardial and mesothelial pleural cells might belong to a population of stem cells. In epithelioid mesotheliomas, 13% of cells expressed nestin, 39% EMA and 7% mesothelin.
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2011.08.009