A PDMS microfluidic impedance immunosensor for E. coli O157:H7 and Staphylococcus aureus detection via antibody-immobilized nanoporous membrane

In this article, a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane was developed for rapid detection of foodborne pathogens Escherichia coli O157:H7 and S taphylococcus aureus with electrochemical impedance spectrum. Firstly, antibodies to the...

Full description

Saved in:
Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2011-11, Vol.159 (1), p.328-335
Main Authors: Tan, Fei, Leung, Polly H.M., Liu, Zong-bin, Zhang, Yu, Xiao, Lidan, Ye, Weiwei, Zhang, Xin, Yi, Li, Yang, Mo
Format: Article
Language:English
Subjects:
Aluminum
aluminum oxide
antibodies
Antibody immobilization
bacteria
Bacteria detection
Electrochemical impedance spectrum
electrochemistry
Escherichia coli
Escherichia coli O157
food pathogens
immunosensors
impedance
microbial detection
Microfluidic immunosensor
Nanoporous membrane
Staphylococcus aureus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this article, a PDMS microfluidic immunosensor integrated with specific antibody immobilized alumina nanoporous membrane was developed for rapid detection of foodborne pathogens Escherichia coli O157:H7 and S taphylococcus aureus with electrochemical impedance spectrum. Firstly, antibodies to the targeted bacteria were covalently immobilized on the nanoporous alumina membranes via self assembled (3-glycidoxypropyl)trimethoxysilane (GPMS) silane. Then, the impedance spectrum was recorded for bacteria detection ranging from 1 Hz to 100 kHz. The maximum impedance amplitude change for these two food pathogens was around 100 Hz. This microfluidic immunosensor based on nanoporous membrane impedance spectrum could achieve rapid bacteria detection within 2 h with a high sensitivity of 10 2 CFU/ml. Cross-bacteria experiments for E. coli O157:H7 and S. aureus were also explored to testify the specificity. The results showed that impedance amplitude at 100 Hz had a significant reduction in binding of bacteria when the membrane was exposed to non-specific bacteria.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2011.06.074