Diabetes increases inflammation and lung injury associated with protective ventilation strategy in mice

• Published in: International immunopharmacology 2012-07, Vol.13 (3), p.280-283
• Main Authors: Xiong, Xiang-qing, Wang, Wan-tie, Wang, Liang-rong, Jin, Li-da, Lin, Li-na
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cytokines - biosynthesis; Cytokines - blood; Cytokines - genetics; Diabetes; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - immunology; Diabetes Mellitus, Experimental - physiopathology; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Inflammation; Inflammation - etiology; Inflammation - immunology; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-6 - genetics; Interleukin-6 - metabolism; Low tidal volume; Lung - immunology; Lung injury; Lung Injury - etiology; Lung Injury - pathology; Lung Injury - physiopathology; Male; Mechanical ventilation; Medical sciences; Mice; Mice, Inbred C57BL; Oxidative Stress; Pharmacology. Drug treatments; Respiration, Artificial - adverse effects; Respiration, Artificial - methods; RNA, Messenger - genetics; RNA, Messenger - metabolism; Tidal Volume; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2012.04.020

Mechanical ventilation may paradoxically cause lung injury. Protective mechanical ventilation strategy utilizing low tidal volume and high frequency has been shown to attenuate inflammation and reduce mortality in non-diabetic patients. The purpose of this present study was to observe the effects of diabetes on inflammation and lung injury in mice with protective ventilation strategy. Forty mice were included in our study. The mice in Group Dia-MV and Con-MV were subjected to 4hour-ventilation. And the mice in Group Dia-SB and Con-SB were exposed to room air breathing spontaneously for 4h. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum were detected and the expression of inflammatory cytokine mRNA was also determined in lung tissue. Lung damage was assessed using a modified lung injury score. The serum levels of TNF-α, IL-6, and IL-10 in Group Dia-MV were significantly higher than those in Group Dia-SB or Group Con-MV or Group Con-SB (P