Copper exposure effects on yeast mitochondrial proteome

Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In thes...

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Bibliographic Details
Published in:Journal of proteomics 2011-10, Vol.74 (11), p.2522-2535
Main Authors: Banci, Lucia, Bertini, Ivano, Ciofi-Baffoni, Simone, D'Alessandro, Annamaria, Jaiswal, Deepa, Marzano, Valeria, Neri, Sara, Ronci, Maurizio, Urbani, Andrea
Format: Article
Language:English
Subjects:
Cell survival
Copper
Data processing
Electrophoresis
Heavy metals
Hydrogen peroxide
LC-MS/MS
Liquid chromatography
Mass spectroscopy
Mitochondria
Organelles
Oxidative stress
Proteomics
Reviews
Saccharomyces cerevisiae
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Summary:Mitochondria play an important role on the entire cellular copper homeostatic mechanisms. Alteration of cellular copper levels may thus influence mitochondrial proteome and its investigation represents an important contribution to the general understanding of copper-related cellular effects. In these study we have performed an organelle targeted proteomic investigation focusing our attention on the effect of non-lethal 1 mM copper concentration on Saccharomyces cerevisiae mitochondrial proteome. Functional copper effects on yeast mitochondrial proteome were evaluated by using both 2D electrophoresis (2-DE) and liquid chromatography coupled with tandem mass spectrometry. Proteomic data have been then analyzed by different unsupervised meta-analysis approaches that highlight the impairment of mitochondrial functions and the activation of oxidative stress response. Interestingly, our data have shown that stress response generated by 1 mM copper treatment determines the activation of S. cerevisiae survival pathway. To investigate these findings we have treated yeast cells responsiveness to copper with hydrogen peroxide and observed a protective role of this metal. These results are suggestive of a copper role in the protection from oxidative stress possibly due to the activation of mechanisms involved in cellular survival and growth. [Display omitted]
ISSN:1874-3919
DOI:10.1016/j.jprot.2011.04.014