Toward single-molecule detection with sensors based on propagating surface plasmons

Surface plasmon resonance (SPR) sensors are known to be able to detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential of SPR biosensors to achieve single-molecule detection, we have minimized the read-out area (to ~64 μm2) by employing a sensor syste...

Full description

Saved in:
Bibliographic Details
Published in:Optics letters 2012-01, Vol.37 (2), p.163-165
Main Authors: Kvasnička, Pavel, Chadt, Karel, Vala, Milan, Bocková, Markéta, Homola, Jiří
Format: Article
Language:English
Subjects:
Biosensors
Collection
Diffraction
Microscopes
Optical Phenomena
Plasmons
Proteins
Sensors
State of the art
Streptavidin - metabolism
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Surface plasmon resonance (SPR) sensors are known to be able to detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential of SPR biosensors to achieve single-molecule detection, we have minimized the read-out area (to ~64 μm2) by employing a sensor system based on spectroscopy of surface plasmons generated on a diffractive structure via a microscope objective and light collection through a small aperture. This approach allows for decreasing the number of detected molecules by 3 orders of magnitude compared to state-of-the-art SPR sensors. A protein monolayer has been shown to produce a response of 5000 times the baseline noise, suggesting that as few as ~500 proteins could be detected by the sensor.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.37.000163