Cytotoxicity of Methylsulfonylmethane on Gastrointestinal (AGS, HepG2, and KEYSE-30) Cancer Cell Lines

Purpose This study was conducted to assay cytotoxic effects of methylsulfonylmethane (MSM) on gastrointestinal cancer cell lines. Methods Human gastric carcinoma (AGS), human hepatocellular carcinoma (HepG2), and human esophageal squamous cell carcinoma (KYSE-30) cancer cell lines were treated by MS...

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Bibliographic Details
Published in:Journal of gastrointestinal cancer 2012-09, Vol.43 (3), p.420-425
Main Authors: Jafari, Naser, Bohlooli, Shahab, Mohammadi, Sadollah, Mazani, Mohammad
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents - pharmacology
Apoptosis - drug effects
Cancer Research
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - pathology
Carcinoma, Squamous Cell - drug therapy
Carcinoma, Squamous Cell - pathology
Cell Cycle - drug effects
Cell Proliferation - drug effects
Dimethyl Sulfoxide - pharmacology
Esophageal Neoplasms - drug therapy
Esophageal Neoplasms - pathology
Flow Cytometry
Gastroenterology
Humans
Internal Medicine
Liver Neoplasms - drug therapy
Liver Neoplasms - pathology
Medicine
Medicine & Public Health
Oncology
Original Article
Radiotherapy
Stomach Neoplasms - drug therapy
Stomach Neoplasms - pathology
Sulfones - pharmacology
Tumor Cells, Cultured
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Summary:Purpose This study was conducted to assay cytotoxic effects of methylsulfonylmethane (MSM) on gastrointestinal cancer cell lines. Methods Human gastric carcinoma (AGS), human hepatocellular carcinoma (HepG2), and human esophageal squamous cell carcinoma (KYSE-30) cancer cell lines were treated by MSM and incubated for 24, 48, and 72 h. Cytotoxicity was examined through MTT, neutral red uptake, and protein measurement assays. Ethidium bromide/acridine orange (EB/AO) staining was used for apoptotic cell detection. A diamidino-2-phenylindole staining method was used to analysis cell cycle by flow cytometry. Results IC 50 of MSM on AGS, HepG2, and KYSE-30 cell lines were 28.04, 21.87 and 27.98 mg/ml after 72 h, respectively. The EB/AO staining showed an increase in apoptotic cells. Cell cycle analysis showed a significant increase in cell density at G2/M phase. Conclusion MSM had cytotoxic effect on cancer cell lines but HepG2 cell line was more susceptible. This study suggests that MSM may induce cytotoxic effect on gastrointestinal cancer cell lines by apoptosis and cell cycle arrest.
ISSN:1941-6628
1941-6636
DOI:10.1007/s12029-011-9291-z