Detection of Anti–β1-AR Autoantibodies in Heart Failure by a Cell-Based Competition ELISA

• Published in: Circulation research 2012-08, Vol.111 (6), p.675-684
• Main Authors: Holthoff, Hans-Peter, Zeibig, Stefan, Jahns-Boivin, Valerie, Bauer, Johannes, Lohse, Martin J, Kääb, Stefan, Clauss, Sebastian, Jahns, Roland, Schlipp, Angela, Münch, Götz, Ungerer, Martin
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibody Affinity - immunology; Antibody Specificity - immunology; Autoantibodies - blood; Autoantibodies - immunology; Base Sequence; Biological and medical sciences; Cardiology. Vascular system; Cardiomyopathy, Dilated - blood; Cardiomyopathy, Dilated - diagnosis; Cardiomyopathy, Dilated - immunology; Enzyme-Linked Immunosorbent Assay - methods; Female; Fundamental and applied biological sciences. Psychology; Heart; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; Humans; Medical sciences; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Myocarditis. Cardiomyopathies; Receptors, Adrenergic, beta-1 - genetics; Receptors, Adrenergic, beta-1 - immunology; Reproducibility of Results; Sensitivity and Specificity; Sf9 Cells; Transfection; Vertebrates: cardiovascular system
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/CIRCRESAHA.112.272682

RATIONALE:Autoantibodies directed against the second extracellular loop of the cardiac β1-adrenergic receptor (β1-AR) are thought to contribute to the pathogenesis of dilated cardiomyopathy (DCM) and Chagas heart disease. Various approaches have been used to detect such autoantibodies; however, the reported prevalence varies largely, depending on the detection method used. OBJECTIVE:We analyzed sera from 167 DCM patients (ejection fraction