Plasma nitrite concentration decreases after hyperoxia-induced oxidative stress in healthy humans

Summary The aim of this study was to measure plasma nitrite, the biochemical marker of endothelial nitric oxide (•NO) synthesis, before and after hyperoxia, in order to test the hypothesis that hyperoxia‐induced vasoconstriction is a consequence of reduced bioavailability of •NO caused by elevated o...

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Bibliographic Details
Published in:Clinical physiology and functional imaging 2012-09, Vol.32 (5), p.404-408
Main Authors: Modun, Darko, Krnic, Mladen, Vukovic, Jonatan, Kokic, Visnja, Kukoc-Modun, Lea, Tsikas, Dimitrios, Dujic, Zeljko
Format: Article
Language:English
Subjects:
Adult
Analysis of Variance
arterial stiffness
Bioavailability
Biological and medical sciences
Biomarkers - blood
Croatia
Down-Regulation
Elevated
endothelium
Fundamental and applied biological sciences. Psychology
Humans
Hyperoxia - blood
Hyperoxia - physiopathology
Imaging
Male
Malondialdehyde - blood
Manometry
Markers
nitric oxide
Nitrites
Nitrites - blood
Oxidative Stress
oxygen
Physiology
Pulse Wave Analysis
Stresses
Time Factors
Vascular Stiffness
Vasoconstriction
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Young Adult
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Summary:Summary The aim of this study was to measure plasma nitrite, the biochemical marker of endothelial nitric oxide (•NO) synthesis, before and after hyperoxia, in order to test the hypothesis that hyperoxia‐induced vasoconstriction is a consequence of reduced bioavailability of •NO caused by elevated oxidative stress. Ten healthy men breathed 100% normobaric O2 for 30 min between 15th and 45th min of the 1‐h study protocol. Plasma nitrite and malondialdehyde (MDA), arterial stiffness (indicated by augmentation index, AIx) and arterial oxygen (PtcO2) pressure were measured at 1st, 15th, 45th and 60th minute of the study. Breathing of normobaric 100% oxygen during 30 min caused an increase in PtcO2 (from 75 ± 2 to 412 ± 25 mm Hg), AIx (from −63 ± 4 to −51 ± 3%) and MDA (from 152 ± 13 to 218 ± 15 nm) values and a decrease in plasma nitrite (from 918 ± 58 to 773 ± 55 nm). During the 15‐min recovery phase, plasma nitrite, AIx and MDA values remained altered. This study suggests that the underlying mechanism of hyperoxia‐induced vasoconstriction may involve reduced •NO bioavailability caused by elevated and sustained oxidative stress.
ISSN:1475-0961
1475-097X
DOI:10.1111/j.1475-097X.2012.01133.x