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Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia

Abstract Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (F...

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Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2012-07, Vol.35 (4), p.319-324
Main Authors: Platell, Joanne L, Cobbold, Rowland N, Johnson, James R, Clabots, Connie R, Trott, Darren J
Format: Article
Language:English
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Summary:Abstract Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQr ) E. coli clinical isolates from humans ( n = 582) and dogs ( n = 120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha , fimH , sat , fyuA , iutA , kpsM II, ompT ). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQr ExPEC population in Australia and may potentially be transferred between humans and dogs.
ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2012.02.002