Binding of polyphenols to plant cell wall analogues – Part 2: Phenolic acids

• Published in: Food chemistry 2012-12, Vol.135 (4), p.2287-2292
• Main Authors: Padayachee, A., Netzel, G., Netzel, M., Day, L., Zabaras, D., Mikkelsen, D., Gidley, M.J.
• Format: Article
• Language: English
• Subjects: Acetobacteraceae - chemistry; Acetobacteraceae - metabolism; bacteria; bioavailability; Biological and medical sciences; carrot juice; carrots; Cell Wall - chemistry; cell walls; Cellulose; Cellulose - chemistry; Cellulose - metabolism; Daucus carota - chemistry; fermentation; Food additives; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; General aspects; Gluconoacetobacter xylinus; Hydroxybenzoates - chemistry; juice concentrates; large intestine; Models, Biological; Pectin; Phenolic acids; Plant cell wall; Plant Preparations - chemistry; plants; Polyphenols; Polyphenols - chemistry; Purple carrot; small intestine
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.07.004

► Interactions of phenolic acids with cellulose and pectin were assessed. ► Chlorogenic, caffeic, and ferulic acids each bound to both polymers. ► Phenolic acid depletion from different juice concentrations was similar. ► Phenolic acids and anthocyanins in purple carrot juice bind similarly to cellulose. Bacterial cellulose and cellulose–pectin composites were used as well-defined model plant cell wall (PCW) systems to study the interaction between phenolic acids (PA) derived from purple carrot juice concentrate (PCJC) and PCW components. Significant PA depletion from solution occurred, with pure cellulose initially (30s–1h) absorbing more than cellulose–pectin composites in the first hour (ca 20% cf 10–15%), but with all composites absorbing similar levels (ca 30%) after several days. Individual PAs bound to different relative extents with caffeic acid>chlorogenic acid>ferulic acid. Extrapolation of data for these model systems to carrot puree suggests that nutritionally-significant amounts of PAs could bind to cell walls, potentially restricting bioavailability in the small intestine and, as a consequence, delivering PAs to the large intestine for fermentation and metabolism by gut bacteria.