Evaluation of dimerization–inhibitory activities of cyclic peptides containing a β-hairpin loop sequence of the EGF receptor

• Published in: Bioorganic & medicinal chemistry 2012-10, Vol.20 (19), p.5730-5737
• Main Authors: Mizuguchi, Takaaki, Ohara, Naho, Iida, Mika, Ninomiya, Ryunosuke, Wada, Shinji, Kiso, Yoshiaki, Saito, Kazuki, Akaji, Kenichi
• Format: Article
• Language: English
• Subjects: A431 cell; Amino Acid Sequence; Biological and medical sciences; Cell Line, Tumor; Cell Proliferation - drug effects; Cyclic peptide; cyclic peptides; Dimerization; EGF receptor; epidermal growth factor receptors; Humans; hydrophobicity; Inhibitor; Medical sciences; Models, Molecular; Molecular Sequence Data; Peptides, Cyclic - chemistry; Peptides, Cyclic - pharmacology; Pharmacology. Drug treatments; Protein Conformation; Protein Multimerization - drug effects; Receptor, Epidermal Growth Factor - antagonists & inhibitors; Receptor, Epidermal Growth Factor - chemistry; Retro-Inverso modification; structure-activity relationships
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/j.bmc.2012.08.013

Structure–activity relationships of cyclic peptides mimicking the β-hairpin structure of the ‘dimerization arm’ at residues 242–259 of the EGF receptor are examined. Cyclic peptides containing the arm head of the β-hairpin loop showed inhibitory activity toward the EGF receptor’s dimerization. Cyclic peptides containing a Retro-Inverso sequence of the dimerization arm showed clear inhibitory effects on the dimerization in vitro and efficiently suppressed the proliferation of A431 cells, which abundantly express the EGF receptor on their surface. The effects at a specific hydrophobic site of the loop structure were expected to enhance the interactions with the receptor.