Peripheral stem cell harvest using regular chemotherapy schedules in childhood cancer

• Published in: Pediatric transplantation 2012-11, Vol.16 (7), p.758-765
• Main Authors: Gooskens, Saskia L., Braakman, Eric, van den Boom, Anne Loes, So-Osman, Cynthia, de Winter, Ferdinand, Pieters, Rob, van den Heuvel-Eibrink, Marry M.
• Format: Article
• Language: English
• Subjects: Adolescent; Antigens, CD34 - biosynthesis; Antineoplastic Agents - administration & dosage; Biological and medical sciences; Blood Component Removal - methods; cancer; Child; Child, Preschool; children; Cohort Studies; Cyclophosphamide - therapeutic use; Female; Filgrastim; General aspects; Granulocyte Colony-Stimulating Factor - therapeutic use; Hematopoietic Stem Cell Mobilization - methods; Humans; Infant; Male; Medical sciences; Neoplasms - therapy; peripheral blood stem cell harvest; Recombinant Proteins - therapeutic use; stem cell mobilization; Stem Cells - cytology; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Time Factors; timing; Treatment Outcome
• ISSN: 1397-3142; 1399-3046
• DOI: 10.1111/j.1399-3046.2012.01754.x

Gooskens SL, Braakman E, van den Boom AL, So‐Osman C, de Winter F, Pieters R, van den Heuvel‐Eibrink MM. Peripheral stem cell harvest using regular chemotherapy schedules in childhood cancer. :  Prediction of the best moment for the harvest of PBSCs after standard chemotherapy followed by filgrastim in children with cancer is difficult. We retrospectively analyzed the moment of harvesting of 152 procedures in 94 patients. The start of apheresis was guided by WBC count and CD34+ cell measurement in peripheral blood. We defined the first day of filgrastim administration, after completion of mobilizing chemotherapy, as day 1. Median time to harvest in different subgroups is as follows: neuroblastoma 11 days (range, 6–29 days), Ewing’s sarcoma nine days (range, 7–15 days), brain tumor 10 days (range, 7–15 days), relapsed Wilms’ tumor 16 days (range, 9–20 days), and extracranial GCT seven days (range, 6–14 days). Patients harvested after cyclophosphamide priming (time to harvest within a range of 8–9 days) were analyzed as a separate group. The optimal moment for harvesting in different types of tumors was highly variable, although most consistent in patients diagnosed with Ewing’s sarcoma or brain tumors and after cyclophosphamide priming.