Enhanced attachment, proliferation, and differentiation of human gingival fibroblasts on titanium surface modified with biomolecules

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2012-11, Vol.100B (8), p.2167-2177
• Main Authors: Jin, Can, Ren, Ling-fei, Ding, Hua-zhu, Shi, Geng-sheng, Lin, Hai-sheng, Zhang, Feng
• Format: Article
• Language: English
• Subjects: Adult; basic fibroblast growth factor; Biological and medical sciences; Cell Adhesion; Cell Differentiation; Cell Proliferation; Cells, Cultured; Coated Materials, Biocompatible - chemistry; Collagen - chemistry; dental implant; Fibroblasts - cytology; Fibroblasts - metabolism; Gingiva - cytology; Gingiva - metabolism; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; human gingival fibroblast; Humans; Hyaluronic Acid - chemistry; Male; Materials Testing; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Oligopeptides - chemistry; RGD peptide; surface modification; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Titanium - chemistry
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.32784

Surface modification of dental implants with biomolecules is of particularly interest recently. To mimic the structure and function of native extracellular matrix (ECM), a derivative of hyaluronic acid (HA), HA–GRGDSP, was synthesized, Arg–Gly–Asp (RGD)‐containing collagen (Col)/HA multilayer polyelectrolyte films (MPFs) coating was fabricated on titanium (Ti) through alternate deposition of Col and HA–GRGDSP with 4.5 assembly cycles; moreover, bioactive molecule, basic fibroblast growth factor (bFGF), was also incorporated into such coating. This coating was then carefully characterized using scanning electronic microscope (SEM) and scanning force microscopy (SFM); bFGF release from the coating was also evaluated. (Col + bFGF)/HA–RGD coating was successfully deposited on Ti surface, and about 300 pg of bFGF could be slowly released from this coating for a week. This coating significantly promoted the initial cell attachment of human gingival fibroblasts (HGFs) compared with other groups (p < 0.05), and HGFs adhered and spread better on this coating than other groups (p < 0.05). Regarding cell proliferation and differentiation of HGFs, they were greatly stimulated when cultured on this coating (p < 0.05). These results indicated that surface modification of Ti using biomolecules might improve the sealing between the neck section of a dental implant and the soft tissue. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.