A Study of High-Altitude Hypoxia-Induced Cell Stress in Murine Model

We evaluated the effects of high-altitude hypoxic stress in the murine model. For this purpose, 36 CR-mice in group A were maintained at the altitude of 3,820 m for hypoxia-induced factor (HIF)-1α expression analysis by immunohistochemistry. The 36 Wistar rats in group B were maintained in low-press...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2012-11, Vol.64 (2), p.85-88
Main Authors: Juan, Hu, Qijun, Wang, Yuanheng, Hu, Yangfang, Li
Format: Article
Language:English
Subjects:
Acclimatization
Altitude
Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Biotin
Cell Biology
Cell Hypoxia
Disease Models, Animal
Endothelin-1 - genetics
Endothelin-1 - metabolism
Female
Gene Expression - drug effects
Heart Atria - drug effects
Heart Atria - metabolism
Heart Ventricles - drug effects
Heart Ventricles - metabolism
Hypoxia
Hypoxia - metabolism
Hypoxia - physiopathology
Hypoxia-Inducible Factor 1, alpha Subunit - genetics
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Life Sciences
Male
Mice
Mitochondria, Heart - drug effects
Mitochondria, Heart - metabolism
Original Paper
Oxygen - metabolism
Oxygen - pharmacology
Pericardium - drug effects
Pericardium - metabolism
Pharmacology/Toxicology
Protein Isoforms - genetics
Protein Isoforms - metabolism
Rats
Rats, Wistar
RNA, Messenger
Stress, Physiological
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
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Summary:We evaluated the effects of high-altitude hypoxic stress in the murine model. For this purpose, 36 CR-mice in group A were maintained at the altitude of 3,820 m for hypoxia-induced factor (HIF)-1α expression analysis by immunohistochemistry. The 36 Wistar rats in group B were maintained in low-pressure (400–420 kPa) oxygen chamber, and the effects of hypoxia on myocardial mitochondria were studied. In the 36 CR-mice of group C, plasma vascular endothelial growth factor (VEGF) levels were determined using strept–avidin–biotin complex/diaminobenzidine method after exposure to different altitudes/O 2 -concentrations. The data show that in experimental group A1, endothelin (ET)-1α concentrations gradually increased whereas HIF-1α expression in myocardial cells was higher ( P  
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-012-9374-x