Comparing culture, real-time PCR and fluorescence resonance energy transfer technology for detection of Porphyromonas gingivalis in patients with or without peri-implant infections

• Published in: Journal of periodontal research 2012-10, Vol.47 (5), p.616-625
• Main Authors: Galassi, F., Kaman, W. E., Anssari Moin, D., van der Horst, J., Wismeijer, D., Crielaard, W., Laine, M. L., Veerman, E. C. I., Bikker, F. J., Loos, B. G.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Bacterial Load; Bacteriological Techniques - statistics & numerical data; Bacteroidaceae Infections - microbiology; Biological and medical sciences; Chromogenic Compounds; d-amino acids; Dental Implants - microbiology; Dental Plaque - microbiology; Dental restorative materials; Dentistry; Feasibility Studies; Female; fluorescence resonance energy transfer; Fluorescence Resonance Energy Transfer - methods; Fluorescence Resonance Energy Transfer - statistics & numerical data; Gingival Hemorrhage - microbiology; Gingival Recession - microbiology; Humans; Infection; Male; Medical sciences; Middle Aged; Otorhinolaryngology. Stomatology; Peri-Implantitis - microbiology; Periodontal Pocket - microbiology; Plaques; Polymerase chain reaction; Porphyromonas gingivalis; Porphyromonas gingivalis - isolation & purification; protease; Real-Time Polymerase Chain Reaction - statistics & numerical data; Saliva; Saliva - microbiology; Sensitivity and Specificity; Stomatitis - microbiology
• ISSN: 0022-3484; 1600-0765
• DOI: 10.1111/j.1600-0765.2012.01474.x

Galassi F, Kaman WE, Anssari Moin D, van der Horst J, Wismeijer D, Crielaard W, Laine ML, Veerman ECI, Bikker FJ, Loos BG. Comparing culture, real‐time PCR and fluorescence resonance energy transfer technology for detection of Porphyromonas gingivalis in patients with or without peri‐implant infections. J Periodont Res 2012; 47: 616–625. © 2012 John Wiley & Sons A/S Background and Objective:  The aim of the study was to compare the detection of Porphyromonas gingivalis using a fluorescence resonance energy transfer (FRET) technology with commonly used diagnostic methods in salivary and subgingival plaque samples from subjects with dental implants. P. gingivalis was considered as a marker for a pathogenic microbiota. Material and Methods:  Ninety‐seven adult subjects were recruited, including periodontally healthy controls with no dental implants, implant controls with no peri‐implant disease and patients with peri‐implant disease. Saliva and subgingival/submucosal plaque samples were collected from all subjects and were analyzed using culture, real‐time PCR and FRET technology employing P. gingivalis‐specific substrates. Results:  It was found that the P. gingivalis‐specific substrates were highly suitable for detecting the presence of P. gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real‐time PCR. Conclusion:  We applied the FRET technology to detect P. gingivalis in implant patients with or without an implant condition and in controls without implants. The technique seems suitable for detection of P. gingivalis in both plaque and saliva samples. However, with all three techniques, P. gingivalis was not very specific for peri‐implantitis cases. Future work includes fine‐tuning the FRET technology and also includes the development of a chair‐side application.