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Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children
Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the ox...
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| Published in: | Life sciences (1973) 2012-11, Vol.91 (19-20), p.907-911 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 911 |
| container_issue | 19-20 |
| container_start_page | 907 |
| container_title | Life sciences (1973) |
| container_volume | 91 |
| creator | Ökrös, Zsuzsanna Endreffy, Emoke Novak, Zoltan Maroti, Zoltan Monostori, Peter Varga, Ilona Sz Király, Agnes Turi, Sandor |
| description | Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment.
Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children.
Higher blood thiobarbituric acid reactive substances (TBARS) (p |
| doi_str_mv | 10.1016/j.lfs.2012.08.039 |
| format | article |
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Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children.
Higher blood thiobarbituric acid reactive substances (TBARS) (p<0.03) and carbonylated protein (p<0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p<0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression.
Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2012.08.039</identifier><identifier>PMID: 22982469</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Administration, Inhalation ; Adolescent ; Adrenergic beta-2 Receptor Agonists - administration & dosage ; Adrenergic beta-2 Receptor Agonists - pharmacology ; Adrenergic beta-2 Receptor Agonists - therapeutic use ; agonists ; antioxidants ; asthma ; Asthma - drug therapy ; Blood ; Bronchial asthma ; Case-Control Studies ; childhood ; children ; Dose-Response Relationship, Drug ; Drug Therapy, Combination ; Female ; gene expression ; Gene Expression Regulation ; Glucocorticoids - administration & dosage ; Glucocorticoids - pharmacology ; Glucocorticoids - therapeutic use ; heme oxygenase (biliverdin-producing) ; Heme Oxygenase-1 - genetics ; Heme Oxygenase-1 - metabolism ; Humans ; inflammation ; Inhaled corticosteroids ; isozymes ; leukocytes ; Leukocytes - metabolism ; Male ; medical treatment ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; messenger RNA ; NADPH oxidase ; NADPH Oxidase 2 ; NADPH Oxidases - genetics ; NADPH Oxidases - metabolism ; Oxidative Stress ; patients ; Polymerase Chain Reaction ; Protein Carbonylation ; quantitative polymerase chain reaction ; RNA, Messenger - metabolism ; Thiobarbituric Acid Reactive Substances - metabolism ; thiobarbituric acid-reactive substances</subject><ispartof>Life sciences (1973), 2012-11, Vol.91 (19-20), p.907-911</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-fd882b59457c951094a400cc2919fde74d963f66944551f48348df6f91c718163</citedby><cites>FETCH-LOGICAL-c377t-fd882b59457c951094a400cc2919fde74d963f66944551f48348df6f91c718163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22982469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ökrös, Zsuzsanna</creatorcontrib><creatorcontrib>Endreffy, Emoke</creatorcontrib><creatorcontrib>Novak, Zoltan</creatorcontrib><creatorcontrib>Maroti, Zoltan</creatorcontrib><creatorcontrib>Monostori, Peter</creatorcontrib><creatorcontrib>Varga, Ilona Sz</creatorcontrib><creatorcontrib>Király, Agnes</creatorcontrib><creatorcontrib>Turi, Sandor</creatorcontrib><title>Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment.
Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children.
Higher blood thiobarbituric acid reactive substances (TBARS) (p<0.03) and carbonylated protein (p<0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p<0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression.
Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.</description><subject>Administration, Inhalation</subject><subject>Adolescent</subject><subject>Adrenergic beta-2 Receptor Agonists - administration & dosage</subject><subject>Adrenergic beta-2 Receptor Agonists - pharmacology</subject><subject>Adrenergic beta-2 Receptor Agonists - therapeutic use</subject><subject>agonists</subject><subject>antioxidants</subject><subject>asthma</subject><subject>Asthma - drug therapy</subject><subject>Blood</subject><subject>Bronchial asthma</subject><subject>Case-Control Studies</subject><subject>childhood</subject><subject>children</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Therapy, Combination</subject><subject>Female</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>Glucocorticoids - administration & dosage</subject><subject>Glucocorticoids - pharmacology</subject><subject>Glucocorticoids - therapeutic use</subject><subject>heme oxygenase (biliverdin-producing)</subject><subject>Heme Oxygenase-1 - genetics</subject><subject>Heme Oxygenase-1 - metabolism</subject><subject>Humans</subject><subject>inflammation</subject><subject>Inhaled corticosteroids</subject><subject>isozymes</subject><subject>leukocytes</subject><subject>Leukocytes - metabolism</subject><subject>Male</subject><subject>medical treatment</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>messenger RNA</subject><subject>NADPH oxidase</subject><subject>NADPH Oxidase 2</subject><subject>NADPH Oxidases - genetics</subject><subject>NADPH Oxidases - metabolism</subject><subject>Oxidative Stress</subject><subject>patients</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Carbonylation</subject><subject>quantitative polymerase chain reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Thiobarbituric Acid Reactive Substances - metabolism</subject><subject>thiobarbituric acid-reactive substances</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kE1vEzEQhi1ERdPAD-ACPnLZZez1flicohRapKqtgJ4txx43jnbXre1U9N_jKIUjlkYejZ95ZT2EvGdQM2Dd5109ulRzYLyGoYZGviILNvSygq5hr8kCgIuq4dCekrOUdgDQtn3zhpxyLgcuOrkgj-utnu8xUT_T69X57SUNv73VCen043pFR3zCkRo90w1SixlNRntgN2MIlupc-q0ey8yEmL0JKWMM3tIcUR9QnfJ20uWFmq0fbcT5LTlxekz47uVekrtvX3-tL6urm4vv69VVZZq-z5Wzw8A3rRRtb2TLQAotAIzhkklnsRdWdo3rOilE2zInhkYM1nVOMtOzgXXNknw65j7E8LjHlNXkk8Fx1DOGfVKMMdFDx4qRJWFH1MSQUkSnHqKfdHxWDNTBtNqpYlodTCsYVDFddj68xO83E9p_G3_VFuDjEXA6KH0ffVJ3P0tCC6V4OYX4ciSwaHjyGFUyHmeD1sciWtng__OBP4F8loY</recordid><startdate>20121102</startdate><enddate>20121102</enddate><creator>Ökrös, Zsuzsanna</creator><creator>Endreffy, Emoke</creator><creator>Novak, Zoltan</creator><creator>Maroti, Zoltan</creator><creator>Monostori, Peter</creator><creator>Varga, Ilona Sz</creator><creator>Király, Agnes</creator><creator>Turi, Sandor</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121102</creationdate><title>Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children</title><author>Ökrös, Zsuzsanna ; Endreffy, Emoke ; Novak, Zoltan ; Maroti, Zoltan ; Monostori, Peter ; Varga, Ilona Sz ; Király, Agnes ; Turi, Sandor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-fd882b59457c951094a400cc2919fde74d963f66944551f48348df6f91c718163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Administration, Inhalation</topic><topic>Adolescent</topic><topic>Adrenergic beta-2 Receptor Agonists - administration & dosage</topic><topic>Adrenergic beta-2 Receptor Agonists - pharmacology</topic><topic>Adrenergic beta-2 Receptor Agonists - therapeutic use</topic><topic>agonists</topic><topic>antioxidants</topic><topic>asthma</topic><topic>Asthma - drug therapy</topic><topic>Blood</topic><topic>Bronchial asthma</topic><topic>Case-Control Studies</topic><topic>childhood</topic><topic>children</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Therapy, Combination</topic><topic>Female</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>Glucocorticoids - administration & dosage</topic><topic>Glucocorticoids - pharmacology</topic><topic>Glucocorticoids - therapeutic use</topic><topic>heme oxygenase (biliverdin-producing)</topic><topic>Heme Oxygenase-1 - genetics</topic><topic>Heme Oxygenase-1 - metabolism</topic><topic>Humans</topic><topic>inflammation</topic><topic>Inhaled corticosteroids</topic><topic>isozymes</topic><topic>leukocytes</topic><topic>Leukocytes - metabolism</topic><topic>Male</topic><topic>medical treatment</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>messenger RNA</topic><topic>NADPH oxidase</topic><topic>NADPH Oxidase 2</topic><topic>NADPH Oxidases - genetics</topic><topic>NADPH Oxidases - metabolism</topic><topic>Oxidative Stress</topic><topic>patients</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Carbonylation</topic><topic>quantitative polymerase chain reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Thiobarbituric Acid Reactive Substances - metabolism</topic><topic>thiobarbituric acid-reactive substances</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ökrös, Zsuzsanna</creatorcontrib><creatorcontrib>Endreffy, Emoke</creatorcontrib><creatorcontrib>Novak, Zoltan</creatorcontrib><creatorcontrib>Maroti, Zoltan</creatorcontrib><creatorcontrib>Monostori, Peter</creatorcontrib><creatorcontrib>Varga, Ilona Sz</creatorcontrib><creatorcontrib>Király, Agnes</creatorcontrib><creatorcontrib>Turi, Sandor</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ökrös, Zsuzsanna</au><au>Endreffy, Emoke</au><au>Novak, Zoltan</au><au>Maroti, Zoltan</au><au>Monostori, Peter</au><au>Varga, Ilona Sz</au><au>Király, Agnes</au><au>Turi, Sandor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2012-11-02</date><risdate>2012</risdate><volume>91</volume><issue>19-20</issue><spage>907</spage><epage>911</epage><pages>907-911</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment.
Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children.
Higher blood thiobarbituric acid reactive substances (TBARS) (p<0.03) and carbonylated protein (p<0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p<0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression.
Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>22982469</pmid><doi>10.1016/j.lfs.2012.08.039</doi><tpages>5</tpages></addata></record> |
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| ispartof | Life sciences (1973), 2012-11, Vol.91 (19-20), p.907-911 |
| issn | 0024-3205 1879-0631 |
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| subjects | Administration, Inhalation Adolescent Adrenergic beta-2 Receptor Agonists - administration & dosage Adrenergic beta-2 Receptor Agonists - pharmacology Adrenergic beta-2 Receptor Agonists - therapeutic use agonists antioxidants asthma Asthma - drug therapy Blood Bronchial asthma Case-Control Studies childhood children Dose-Response Relationship, Drug Drug Therapy, Combination Female gene expression Gene Expression Regulation Glucocorticoids - administration & dosage Glucocorticoids - pharmacology Glucocorticoids - therapeutic use heme oxygenase (biliverdin-producing) Heme Oxygenase-1 - genetics Heme Oxygenase-1 - metabolism Humans inflammation Inhaled corticosteroids isozymes leukocytes Leukocytes - metabolism Male medical treatment Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism messenger RNA NADPH oxidase NADPH Oxidase 2 NADPH Oxidases - genetics NADPH Oxidases - metabolism Oxidative Stress patients Polymerase Chain Reaction Protein Carbonylation quantitative polymerase chain reaction RNA, Messenger - metabolism Thiobarbituric Acid Reactive Substances - metabolism thiobarbituric acid-reactive substances |
| title | Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children |
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