Fructophilic Lactobacillus kunkeei and Lactobacillus brevis Isolated from Fresh Flowers, Bees and Bee-hives

Two-hundred-and-thirty-six isolates were collected from fresh flowers, bees and bee-hives. Of these, 20 isolates preferred D-fructose as carbon source, produced lactic acid and acetic acid but trace amounts of ethanol and were classified as fructophilic. Poor growth was recorded when strains were in...

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Bibliographic Details
Published in:Current microbiology 2012-11, Vol.65 (5), p.507-515
Main Authors: Neveling, Deon P, Endo, Akihito, Dicks, Leon M. T
Format: Article
Language:English
Subjects:
Acetic acid
Animals
Apoidea
Bacterial Typing Techniques
Bacteriology
Bees
Bees - microbiology
Biomedical and Life Sciences
Biotechnology
carbon
Carbon sources
Ethanol
flowers
Flowers & plants
Flowers - microbiology
fructose
Glucose - metabolism
Indexing in process
lactic acid
Lactobacillus - classification
Lactobacillus - genetics
Lactobacillus - isolation & purification
Lactobacillus - metabolism
Lactobacillus brevis
Lactobacillus brevis - classification
Lactobacillus brevis - genetics
Lactobacillus brevis - isolation & purification
Lactobacillus brevis - metabolism
Lactobacillus kunkeei
Life Sciences
Microbiology
Molecular Sequence Data
nucleotide sequences
oxygen
Phylogeny
pyruvic acid
ribosomal RNA
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Summary:Two-hundred-and-thirty-six isolates were collected from fresh flowers, bees and bee-hives. Of these, 20 isolates preferred D-fructose as carbon source, produced lactic acid and acetic acid but trace amounts of ethanol and were classified as fructophilic. Poor growth was recorded when strains were incubated anaerobically in the presence of D-glucose as sole carbon source. Good growth was, however, recorded when D-glucose was metabolized in the presence of external electron acceptors such as fructose, pyruvate and oxygen. Nineteen of the strains were classified as Lactobacillus kunkeei and one as Lactobacillus brevis based on phenotypic characteristics, 16S rRNA sequences, recA sequences and DNA homology. This is the first description of a fructophilic strain of L. brevis.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-012-0186-4