Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells

► Spinach aqueous extract suppressed antigen-induced degranulation of RBL-2H3 cells. ► Active substances in the extract were heat-stable and trypsin-resistant. ► The molecular weights of the active substances ranged from 500Da to 14kDa. ► The extract suppressed degranulation by decreased Ca2+ influx...

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Bibliographic Details
Published in:Food chemistry 2013-01, Vol.136 (2), p.322-327
Main Authors: Ishida, Momoko, Nishi, Kosuke, Watanabe, Hisashi, Sugahara, Takuya
Format: Article
Language:English
Subjects:
Animals
Anti-allergic activity
Anti-Allergic Agents - pharmacology
Basophils - drug effects
Basophils - immunology
Biological and medical sciences
Calcium - immunology
Cell Degranulation - drug effects
Cell Line, Tumor
Degranulation
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Plant Extracts - pharmacology
Rats
RBL-2H3 cell
Spinach
Spinacia oleracea - chemistry
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Summary:► Spinach aqueous extract suppressed antigen-induced degranulation of RBL-2H3 cells. ► Active substances in the extract were heat-stable and trypsin-resistant. ► The molecular weights of the active substances ranged from 500Da to 14kDa. ► The extract suppressed degranulation by decreased Ca2+ influx into the cells. ► Suppressed Ca2+ influx seems to result from decreased phosphorylation of Syk and PI3K. The inhibitory effect of an aqueous extract from spinach on degranulation of RBL-2H3 cells is herein reported. The extract significantly suppressed antigen-induced degranulation in a dose-dependent manner without affecting cell viability. Active substances in the extract were heat-stable and trypsin-resistant with molecular weights ranging from 500Da to 14kDa. The extract inhibited elevation of the intracellular Ca2+ concentration caused by stimulation by antigen, while not suppressing degranulation induced by a calcium ionophore A23187. Immunoblot analysis revealed that the inhibitory effect results from downregulation of phosphorylation of both Syk kinase and phosphatidylinositol 3-kinase in the signalling pathways involved in degranulation caused by the antigen–antibody interaction. Taken together, these findings suggest that aqueous spinach extract has an anti-allergic activity that controls degranulation.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2012.08.079