Defense peptides from barnyard grass (Echinochloa crusgalli L.) seeds

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2012-11, Vol.38 (1), p.33-40
• Main Authors: Rogozhin, E.A., Ryazantsev, D.Y., Grishin, E.V., Egorov, T.A., Zavriev, S.K.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Anti-Bacterial Agents - pharmacology; Antifungal Agents - pharmacology; antimicrobial peptides; Base Sequence; bioactive properties; Chromatography, High Pressure Liquid - methods; Echinochloa - chemistry; Echinochloa crus-galli; Echinochloa crusgalli seeds; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - pathogenicity; Gram-Positive Bacteria - drug effects; Gram-Positive Bacteria - pathogenicity; High performance liquid chromatography; matrix-assisted laser desorption-ionization mass spectrometry; Microbial Sensitivity Tests; Molecular Sequence Data; peptides; Phytophthora infestans; Phytophthora infestans - drug effects; Phytophthora infestans - pathogenicity; Plant defense peptides; Plant Diseases - microbiology; Plant immunity; plant pathogenic bacteria; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; Plant Proteins - pharmacology; polypeptides; protease inhibitors; Protease Inhibitors - chemistry; Protease Inhibitors - pharmacology; proteinase inhibitors; seed germination; seeds; Seeds - chemistry; Solanum tuberosum - microbiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; weeds
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2012.08.009

► A spectrum of novel defense peptides was isolated from Echinochloa crusgalli seeds. ► N-terminal primary sequences of these peptides were determined and it was shown that these molecules belong to cystein-rich plant peptides. ► There are peptides from three AMP families and two protease inhibitor families in barnyard grass seeds. ► The biological activity of isolated peptides against several phytopatogenic bacterial species and oomycete Phytophthora infestans was studied. A number of defense polypeptides from latent seeds of weed cereal barnyard grass (Echinochloa crusgalli L.) has been isolated and characterized using an acidic extraction and high performance liquid chromatography methods in combination with MALDI-TOF mass spectrometry and Edman sequencing. Members of three antimicrobial peptide families and two protease inhibitor families were found to be localized in barnyard grass seeds. Their biological activity concerning to Gram-Positive and Gram-Negative phytopathogenic bacteria, as well as oomycete Phytophthora infestans, has been investigated. Diversity of barnyard grass defense peptides is a significant factor that provides a resistance of E. crusgalli seeds to germination and latent phases.