Nucleotide polymorphisms related to altitude and physiological traits in contrasting provenances of Norway spruce (Picea abies)

Variation of sequences of six EST-derived markers was investigated in three Norway spruce (Picea abies [L.] Karst.) provenances originating from different altitudes growing at two contrasting trial plots in Slovakia (Veľký Lom 450 m a.s.l., Mútne-Zákamenné 1,250 m a.s.l.) within a spin-off experimen...

Full description

Saved in:
Bibliographic Details
Published in:Biológia 2012-10, Vol.67 (5), p.909-916
Main Authors: Romšáková, Ivana, Foffová, Elena, Kmeť, Jaroslav, Longauer, Roman, Pacalaj, Marian, Gömöry, Dušan
Format: Article
Language:English
Subjects:
Adaptations
Altitude
Biomedical and Life Sciences
Cell Biology
Chlorophyll
chlorophyll fluorescence
cold hardiness
Differentiation
Fluorescence
Frost
frost resistance
Gene frequency
growth traits
Inventories
IUFRO 1964/68
Life Sciences
Microbiology
Phenology
Picea abies
Plant Sciences
provenance
provenance research
Section Botany
single nucleotide polymorphisms
Single-nucleotide polymorphism
variance
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Variation of sequences of six EST-derived markers was investigated in three Norway spruce (Picea abies [L.] Karst.) provenances originating from different altitudes growing at two contrasting trial plots in Slovakia (Veľký Lom 450 m a.s.l., Mútne-Zákamenné 1,250 m a.s.l.) within a spin-off experiment of the IUFRO 1964/68 Inventory Provenance Experiment with Norway spruce. Single nucleotide polymorphisms (SNP) were identified and differences in allele frequencies at polymorphic sites were tested against altitude or associated with physiological and growth traits (chlorophyll a fluorescence, frost resistance, height, diameter, budburst phenology).Overall, 5.1% of sites (190 in total) were polymorphic in the studied material. Although there were no differences in nucleotide diversity among provenances, the differentiation was highly significant (the overall between-population variance component assessed by the AMOVA based on both extreme populations P1 and P49 was 6.53%). Only 4 polymorphic sites differed significantly between populations after Bonferroni correction. Four sites showed significant association with phenotypic traits (breast-height diameter, stem volume, chlorophyll fluorescence). In contrast to earlier analyses of growth and physiological traits based on the same material, significant associations with polymorphic sites indicate the effect of local adaptation.
ISSN:0006-3088
1336-9563
DOI:10.2478/s11756-012-0077-y